cad a22 4500 31694954X 20181103043304.0 cr unu---uuuuu 140620e20140402xx s 000 0 eng 10.5167/uzh-96600 doi 10.1152/ajpregu.00533.2013 doi (ZORA)oai:www.zora.uzh.ch:96600 570 ddc 610 ddc 24694381 pmid Interleukin-6 contributes to early fasting-induced free fatty acid mobilization in mice [Elektronische Daten] [Stephan Wueest, Flurin Item, Christina N Boyle, Paulin Jirkof, Nikola Cesarovic, Helga Ellingsgaard, Marianne Böni-Schnetzler, Katharina Timper, Margarete Arras, Marc Y Donath, Thomas A Lutz, Eugen J Schoenle, Daniel Konrad] closedAccess eu-repo Contracting muscle releases interleukin-6 (IL-6) enabling the metabolic switch from carbohydrate to fat utilization. Similarly, metabolism is switched during transition from fed to fasting state. Herein, we examined a putative role for IL-6 in the metabolic adaptation to normal fasting. In lean C57BL/6J mice, 6 h of food withdrawal increased gene transcription levels of IL-6 in skeletal muscle but not in white adipose tissue. Concomitantly, circulating IL-6 and free fatty acid (FFA) levels were significantly increased, whereas respiratory quotient (RQ) was reduced in 6-h fasted mice. In white adipose tissue, phosphorylation of hormone-sensitive lipase (HSL) was increased on fasting, indicating increased lipolysis. Intriguingly, fasting-induced increase in circulating IL-6 levels and parallel rise in FFA concentration were absent in obese and glucose-intolerant mice. A causative role for IL-6 in the physiological adaptation to fasting was further supported by the fact that fasting-induced increase in circulating FFA levels was significantly blunted in lean IL-6 knockout (KO) and lean C57BL/6J mice treated with neutralizing IL-6 antibody. Consistently, phosphorylation of HSL was significantly reduced in adipose tissue of IL-6-depleted mice. Hence, our findings suggest a novel role for IL-6 in energy supply during early fasting. Division of Surgical Research zora Medical Clinic zora Center for Integrative Human Physiology zora Institute of Veterinary Physiology zora Institute of Laboratory Animal Science zora Wueest Stephan joint author Item Flurin joint author Boyle Christina N. joint author Jirkof Paulin joint author Cesarovic Nikola joint author Ellingsgaard Helga joint author Böni-Schnetzler Marianne joint author Timper Katharina joint author Arras Margarete joint author Donath Marc Y. joint author Lutz Thomas A. joint author Schoenle Eugen J. joint author Konrad Daniel joint author American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 306(11):R861-R867 https://www.zora.uzh.ch/id/eprint/96600/1/Interleukin-6_VET-Apr2-2014.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/96600/1/Interleukin-6_VET-Apr2-2014.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Wueest Stephan joint author ZORA 700 1- Item Flurin joint author ZORA 700 1- Boyle Christina N. joint author ZORA 700 1- Jirkof Paulin joint author ZORA 700 1- Cesarovic Nikola joint author ZORA 700 1- Ellingsgaard Helga joint author ZORA 700 1- Böni-Schnetzler Marianne joint author ZORA 700 1- Timper Katharina joint author ZORA 700 1- Arras Margarete joint author ZORA 700 1- Donath Marc Y. joint author ZORA 700 1- Lutz Thomas A. joint author ZORA 700 1- Schoenle Eugen J. joint author ZORA 700 1- Konrad Daniel joint author ZORA 773 0- American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 306(11):R861-R867 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article closedAccess