cad a22 4500 317037137 20200528035006.0 cr unu---uuuuu 140621s2014 xx s 000 0 eng 10.5167/uzh-96639 doi 10.1111/jvim.12334 doi (ZORA)oai:www.zora.uzh.ch:96639 570 ddc 610 ddc 24597630 pmid Agreement of serum Spec cPL with the 1,2-o-dilauryl-rac-glycero glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay and with pancreatic ultrasonography in dogs with suspected pancreatitis [Elektronische Daten] [Peter H Kook, N Kohler, Sonja Hartnack, Barbara Riond, Claudia E Reusch] closedAccess eu-repo BACKGROUND: Spec cPL is the most sensitive and specific test for diagnosing pancreatitis in dogs. Its results have not been compared to those of the 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay or those of abdominal ultrasonography. OBJECTIVES: To investigate agreement of Spec cPL with DGGR lipase activity and pancreatic ultrasonography in dogs with suspected pancreatitis. ANIMALS: One hundred and forty-two dogs. METHODS: DGGR lipase activity (reference range, 24-108 U/L) and Spec cPL were measured using the same sample. The time interval between ultrasonography and lipase determinations was <24 hours. The agreement of the 2 lipase assays at different cutoffs and the agreement between pancreatic ultrasonography and the 2 tests were assessed using Cohen's kappa coefficient (κ). RESULTS: DGGR lipase (>108, >216 U/L) and Spec cPL (>200 μg/L) had κ values of 0.79 (95% confidence interval [CI], 0.69-0.9) and 0.70 (CI, 0.58-0.82). DGGR lipase (>108, >216 U/L) and Spec cPL (>400 μg/L) had κ values of 0.55 (CI, 0.43-0.67) and κ of 0.80 (CI, 0.71-0.9). An ultrasonographic diagnosis of pancreatitis and DGGR lipase (>108, >216 U/L) had κ values of 0.29 (CI, 0.14-0.44) and 0.35 (CI, 0.18-0.52). Ultrasonographically diagnosed pancreatitis and Spec cPL (>200, >400 μg/L) had κ values of 0.25 (CI, 0.08-0.41) and 0.27 (CI, 0.09-0.45). CONCLUSIONS AND CLINICAL IMPORTANCE: Although both lipase assays showed high agreement, agreement between ultrasonography and lipase assays results was only fair. Because lipase results are deemed more accurate, ultrasonography results should be interpreted carefully. Department of Farm Animals zora Department of Small Animals zora Chair in Veterinary Epidemiology zora Kook Peter H. joint author Kohler N. joint author Hartnack Sonja joint author Riond Barbara joint author Reusch Claudia E. joint author Journal of Veterinary Internal Medicine 28(3):863-870 https://www.zora.uzh.ch/id/eprint/96639/1/Kook_2014_JVIM.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/96639/1/Kook_2014_JVIM.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Kook Peter H. joint author ZORA 700 1- Kohler N. joint author ZORA 700 1- Hartnack Sonja joint author ZORA 700 1- Riond Barbara joint author ZORA 700 1- Reusch Claudia E. joint author ZORA 773 0- Journal of Veterinary Internal Medicine 28(3):863-870 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article closedAccess