caa a22 4500 357790162 20200908030107.0 cr unu---uuuuu 160305s2015 xx s 000 0 eng (BORIS)oai:boris.unibe.ch:76344 570 ddc 610 ddc Flagellar membranes are rich in raft-forming phospholipids [Elektronische Daten] [Mauro Serricchio, Adrien W Schmid, Michael Eduard Steinmann, Erwin Sigel, Monika Rauch, Daria Julkowska, Serge Bonnefoy, Cécile Fort, Philippe Bastin, Peter Bütikofer] publishedVersion openAccess eu-repo The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei. Serricchio Mauro joint author Schmid Adrien W. joint author Steinmann Michael Eduard joint author Sigel Erwin joint author Rauch Monika joint author Julkowska Daria joint author Bonnefoy Serge joint author Fort Cécile joint author Bastin Philippe joint author Bütikofer Peter joint author Biology open BiO 4(9), pp. 1143-1153 Onlinezugriff via WWW text/html Serricchio, Mauro; Schmid, Adrien W; Steinmann, Michael Eduard; Sigel, Erwin; Rauch, Monika; Julkowska, Daria; Bonnefoy, Serge; Fort, Cécile; Bastin, Philippe; Bütikofer, Peter (2015). Flagellar membranes are rich in raft-forming phospholipids. Biology open BiO, 4(9), pp. 1143-1153. Company of Biologists 10.1242/bio.011957 <http://dx.doi.org/10.1242/bio.011957> BORIS BK010053 XK010053 XK010000 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS BORIS BORIS info:eu-repo/semantics/article openAccess BORIS 856 42 Onlinezugriff via WWW text/html Serricchio, Mauro; Schmid, Adrien W; Steinmann, Michael Eduard; Sigel, Erwin; Rauch, Monika; Julkowska, Daria; Bonnefoy, Serge; Fort, Cécile; Bastin, Philippe; Bütikofer, Peter (2015). Flagellar membranes are rich in raft-forming phospholipids. Biology open BiO, 4(9), pp. 1143-1153. Company of Biologists 10.1242/bio.011957 <http://dx.doi.org/10.1242/bio.011957> BORIS 700 1- Serricchio Mauro joint author BORIS 700 1- Schmid Adrien W. joint author BORIS 700 1- Steinmann Michael Eduard joint author BORIS 700 1- Sigel Erwin joint author BORIS 700 1- Rauch Monika joint author BORIS 700 1- Julkowska Daria joint author BORIS 700 1- Bonnefoy Serge joint author BORIS 700 1- Fort Cécile joint author BORIS 700 1- Bastin Philippe joint author BORIS 700 1- Bütikofer Peter joint author BORIS 773 0- Biology open BiO 4(9), pp. 1143-1153