caa a22 4500 357790197 20170617031055.0 cr unu---uuuuu 160305s2015 xx s 000 0 eng 2090-2247 10.1155/2015/617620 doi (BORIS)oai:boris.unibe.ch:76359 570 ddc 610 ddc 26509084 pmid Modulatory Effects of Eschscholzia californica Alkaloids on Recombinant GABAA Receptors [Elektronische Daten] [Milan Fedurco, Jana Gregorová, Kristýna Šebrlová, Jana Kantorová, Ondřej Peš, Roland Baur, Erwin Sigel, Eva Táborská] publishedVersion openAccess eu-repo The California poppy (Eschscholzia californica Cham.) contains a variety of natural compounds including several alkaloids found exclusively in this plant. Because of the sedative, anxiolytic, and analgesic effects, this herb is currently sold in pharmacies in many countries. However, our understanding of these biological effects at the molecular level is still lacking. Alkaloids detected in E. californica could be hypothesized to act at GABAA receptors, which are widely expressed in the brain mainly at the inhibitory interneurons. Electrophysiological studies on a recombinant α 1 β 2 γ 2 GABAA receptor showed no effect of N-methyllaurotetanine at concentrations lower than 30 μM. However, (S)-reticuline behaved as positive allosteric modulator at the α 3, α 5, and α 6 isoforms of GABAA receptors. The depressant properties of aerial parts of E. californica are assigned to chloride-current modulation by (S)-reticuline at the α 3 β 2 γ 2 and α 5 β 2 γ 2 GABAA receptors. Interestingly, α 1, α 3, and α 5 were not significantly affected by (R)-reticuline, 1,2-tetrahydroreticuline, codeine, and morphine-suspected (S)-reticuline metabolites in the rodent brain. Fedurco Milan joint author Gregorová Jana joint author Šebrlová Kristýna joint author Kantorová Jana joint author Peš Ondřej joint author Baur Roland joint author Sigel Erwin joint author Táborská Eva joint author Biochemistry research international 2015(617620), p. 617620 2090-2247 http://boris.unibe.ch/76359/1/Sigel%20Modulatory%20Effect%20of%20Eschscholzia.pdf text/html WWW-Backlink auf das Repository 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS 856 40 http://boris.unibe.ch/76359/1/Sigel%20Modulatory%20Effect%20of%20Eschscholzia.pdf text/html WWW-Backlink auf das Repository BORIS 700 1- Fedurco Milan joint author BORIS 700 1- Gregorová Jana joint author BORIS 700 1- Šebrlová Kristýna joint author BORIS 700 1- Kantorová Jana joint author BORIS 700 1- Peš Ondřej joint author BORIS 700 1- Baur Roland joint author BORIS 700 1- Sigel Erwin joint author BORIS 700 1- Táborská Eva joint author BORIS 773 0- Biochemistry research international 2015(617620), p. 617620 2090-2247 BK010053 XK010053 XK010000 BORIS BORIS BORIS info:eu-repo/semantics/article openAccess