caa a22 4500 357880099 20170617031055.0 cr unu---uuuuu 160308e201502 xx s 000 0 eng 0887-6924 10.1038/leu.2014.217 doi (BORIS)oai:boris.unibe.ch:78793 610 ddc 25036192 pmid A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real-time quantitative PCR [Elektronische Daten] [H White, L Deprez, P Corbisier, V Hall, F Lin, S Mazoua, S Trapmann, A Aggerholm, H Andrikovics, S Akiki, G Barbany, N Boeckx, A Bench, M Catherwood, J-M Cayuela, S Chudleigh, T Clench, D Colomer, F Daraio, S Dulucq, J Farrugia, L Fletcher, L Foroni, R Ganderton, G Gerrard, E Gineikienė, S Hayette, H El Housni, B Izzo, M Jansson, P Johnels, T Jurcek, V Kairisto, A Kizilors, D-W Kim, T Lange, T Lion, K M Polakova, G Martinelli, S McCarron, P A Merle, B Milner, G Mitterbauer-Hohendanner, M Nagar, G Nickless, J Nomdedéu, D A Nymoen, Elisabeth Oppliger Leibundgut, U Ozbek, T Pajič, H Pfeifer, C Preudhomme, K Raudsepp, G Romeo, T Sacha, R Talmaci, T Touloumenidou, V H J Van der Velden, P Waits, L Wang, E Wilkinson, G Wilson, D Wren, R Zadro, J Ziermann, K Zoi, M C Müller, A Hochhaus, H Schimmel, N C P Cross, H Emons] publishedVersion openAccess eu-repo Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 10(6), 1.08±0.11 × 10(5), 1.03±0.10 × 10(4), 1.02±0.09 × 10(3), 1.04±0.10 × 10(2) and 10.0±1.5 copies/μl. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR-ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR-ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec.europa.eu/jrc/en/reference-materials/catalogue/; CRM code ERM-AD623a-f). White H. joint author Deprez L. joint author Corbisier P. joint author Hall V. joint author Lin F. joint author Mazoua S. joint author Trapmann S. joint author Aggerholm A. joint author Andrikovics H. joint author Akiki S. joint author Barbany G. joint author Boeckx N. joint author Bench A. joint author Catherwood M. joint author Cayuela J-M joint author Chudleigh S. joint author Clench T. joint author Colomer D. joint author Daraio F. joint author Dulucq S. joint author Farrugia J. joint author Fletcher L. joint author Foroni L. joint author Ganderton R. joint author Gerrard G. joint author Gineikienė E. joint author Hayette S. joint author El Housni H. joint author Izzo B. joint author Jansson M. joint author Johnels P. joint author Jurcek T. joint author Kairisto V. joint author Kizilors A. joint author Kim D-W joint author Lange T. joint author Lion T. joint author Polakova K M. joint author Martinelli G. joint author McCarron S. joint author Merle P A. joint author Milner B. joint author Mitterbauer-Hohendanner G. joint author Nagar M. joint author Nickless G. joint author Nomdedéu J. joint author Nymoen D A. joint author Oppliger Leibundgut Elisabeth joint author Ozbek U. joint author Pajič T. joint author Pfeifer H. joint author Preudhomme C. joint author Raudsepp K. joint author Romeo G. joint author Sacha T. joint author Talmaci R. joint author Touloumenidou T. joint author Van der Velden V H J. joint author Waits P. joint author Wang L. joint author Wilkinson E. joint author Wilson G. joint author Wren D. joint author Zadro R. joint author Ziermann J. joint author Zoi K. joint author Müller M C. joint author Hochhaus A. joint author Schimmel H. joint author Cross N C P. joint author Emons H. joint author Leukemia 29(2), pp. 369-376 0887-6924 http://boris.unibe.ch/78793/1/EO_leu2014217a.pdf text/html WWW-Backlink auf das Repository 1 info:eu-repo/semantics/article boris PeerReviewed boris peerstatus BORIS 856 40 http://boris.unibe.ch/78793/1/EO_leu2014217a.pdf text/html WWW-Backlink auf das Repository BORIS 700 1- White H. joint author BORIS 700 1- Deprez L. joint author BORIS 700 1- Corbisier P. joint author BORIS 700 1- Hall V. joint author BORIS 700 1- Lin F. joint author BORIS 700 1- Mazoua S. joint author BORIS 700 1- Trapmann S. joint author BORIS 700 1- Aggerholm A. joint author BORIS 700 1- Andrikovics H. joint author BORIS 700 1- Akiki S. joint author BORIS 700 1- Barbany G. joint author BORIS 700 1- Boeckx N. joint author BORIS 700 1- Bench A. joint author BORIS 700 1- Catherwood M. joint author BORIS 700 1- Cayuela J-M joint author BORIS 700 1- Chudleigh S. joint author BORIS 700 1- Clench T. joint author BORIS 700 1- Colomer D. joint author BORIS 700 1- Daraio F. joint author BORIS 700 1- Dulucq S. joint author BORIS 700 1- Farrugia J. joint author BORIS 700 1- Fletcher L. joint author BORIS 700 1- Foroni L. joint author BORIS 700 1- Ganderton R. joint author BORIS 700 1- Gerrard G. joint author BORIS 700 1- Gineikienė E. joint author BORIS 700 1- Hayette S. joint author BORIS 700 1- El Housni H. joint author BORIS 700 1- Izzo B. joint author BORIS 700 1- Jansson M. joint author BORIS 700 1- Johnels P. joint author BORIS 700 1- Jurcek T. joint author BORIS 700 1- Kairisto V. joint author BORIS 700 1- Kizilors A. joint author BORIS 700 1- Kim D-W joint author BORIS 700 1- Lange T. joint author BORIS 700 1- Lion T. joint author BORIS 700 1- Polakova K M. joint author BORIS 700 1- Martinelli G. joint author BORIS 700 1- McCarron S. joint author BORIS 700 1- Merle P A. joint author BORIS 700 1- Milner B. joint author BORIS 700 1- Mitterbauer-Hohendanner G. joint author BORIS 700 1- Nagar M. joint author BORIS 700 1- Nickless G. joint author BORIS 700 1- Nomdedéu J. joint author BORIS 700 1- Nymoen D A. joint author BORIS 700 1- Oppliger Leibundgut Elisabeth joint author BORIS 700 1- Ozbek U. joint author BORIS 700 1- Pajič T. joint author BORIS 700 1- Pfeifer H. joint author BORIS 700 1- Preudhomme C. joint author BORIS 700 1- Raudsepp K. joint author BORIS 700 1- Romeo G. joint author BORIS 700 1- Sacha T. joint author BORIS 700 1- Talmaci R. joint author BORIS 700 1- Touloumenidou T. joint author BORIS 700 1- Van der Velden V H J. joint author BORIS 700 1- Waits P. joint author BORIS 700 1- Wang L. joint author BORIS 700 1- Wilkinson E. joint author BORIS 700 1- Wilson G. joint author BORIS 700 1- Wren D. joint author BORIS 700 1- Zadro R. joint author BORIS 700 1- Ziermann J. joint author BORIS 700 1- Zoi K. joint author BORIS 700 1- Müller M C. joint author BORIS 700 1- Hochhaus A. joint author BORIS 700 1- Schimmel H. joint author BORIS 700 1- Cross N C P. joint author BORIS 700 1- Emons H. joint author BORIS 773 0- Leukemia 29(2), pp. 369-376 0887-6924 BK010053 XK010053 XK010000 BORIS BORIS BORIS info:eu-repo/semantics/article openAccess