caa a22 4500 357894472 CHVBK 20170617160604.0 cr unu---uuuuu 160308e201602 xx s 000 0 eng 10.1007/s00572-015-0657-0 doi (SERVAL)BIB_B251BA4088C9 26208816 pmid Mycorrhization between Cistus ladanifer L. and Boletus edulis Bull is enhanced by the mycorrhiza helper bacteria Pseudomonas fluorescens Migula [Elektronische Daten] [O. Mediavilla, J. Olaizola, L. Santos-del-Blanco, J.A. Oria-de-Rueda, P. Martín-Pinto] Boletus edulis Bull. is one of the most economically and gastronomically valuable fungi worldwide. Sporocarp production normally occurs when symbiotically associated with a number of tree species in stands over 40years old, but it has also been reported in 3-year-old Cistus ladanifer L. shrubs. Efforts toward the domestication of B. edulis have thus focused on successfully generating C. ladanifer seedlings associated with B. edulis under controlled conditions. Microorganisms have an important role mediating mycorrhizal symbiosis, such as some bacteria species which enhance mycorrhiza formation (mycorrhiza helper bacteria). Thus, in this study, we explored the effect that mycorrhiza helper bacteria have on the efficiency and intensity of the ectomycorrhizal symbiosis between C. ladanifer and B. edulis. The aim of this work was to optimize an in vitro protocol for the mycorrhizal synthesis of B. edulis with C. ladanifer by testing the effects of fungal culture time and coinoculation with the helper bacteria Pseudomonas fluorescens Migula. The results confirmed successful mycorrhizal synthesis between C. ladanifer and B. edulis. Coinoculation of B. edulis with P. fluorescens doubled within-plant mycorrhization levels although it did not result in an increased number of seedlings colonized with B. edulis mycorrhizae. B. edulis mycelium culture time also increased mycorrhization levels but not the presence of mycorrhizae. These findings bring us closer to controlled B. edulis sporocarp production in plantations. Mediavilla O. aut Olaizola J. aut Santos-del-Blanco L. aut Oria-de-Rueda J.A. aut Martín-Pinto P. aut Mycorrhiza 26/2(2016-02), 161-168 26:2<161-168 2016 26 1 article serval SERVAL 700 1- Mediavilla O. aut SERVAL 700 1- Olaizola J. aut SERVAL 700 1- Santos-del-Blanco L. aut SERVAL 700 1- Oria-de-Rueda J.A. aut SERVAL 700 1- Martín-Pinto P. aut SERVAL 773 0- Mycorrhiza 26/2(2016-02), 161-168 26:2<161-168 2016 26 BK010053 XK010053 XK010000 SERVAL SERVAL SERVAL article