cad a22 4500 357963652 20181103044140.0 cr unu---uuuuu 160310s2016 xx s 000 0 eng 10.5167/uzh-123280 doi 10.1038/srep21377 doi (ZORA)oai:www.zora.uzh.ch:123280 570 ddc 610 ddc 26903281 pmid T cell-specific inactivation of mouse CD2 by CRISPR/Cas9 [Elektronische Daten] [Jane Beil-Wagner, Georg Dössinger, Kilian Schober, Johannes vom Berg, Achim Tresch, Martina Grandl, Pushpalatha Palle, Florian Mair, Markus Gerhard, Burkhard Becher, Dirk H Busch, Thorsten Buch] openAccess eu-repo The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4+ and CD8+ lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific promoters can edit genes in the mouse and may thus allow targeted studies of gene function in vivo. Institute of Laboratory Animal Science zora Institute of Experimental Immunology zora Beil-Wagner Jane joint author Dössinger Georg joint author Schober Kilian joint author vom Berg Johannes joint author Tresch Achim joint author Grandl Martina joint author Palle Pushpalatha joint author Mair Florian joint author Gerhard Markus joint author Becher Burkhard joint author Busch Dirk H. joint author Buch Thorsten joint author Scientific Reports 6:21377 https://www.zora.uzh.ch/id/eprint/123280/1/Nature%20sci%20rep%20Jane%20Beil-Wagner%20et%20al.%202016.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/123280/1/Nature%20sci%20rep%20Jane%20Beil-Wagner%20et%20al.%202016.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Beil-Wagner Jane joint author ZORA 700 1- Dössinger Georg joint author ZORA 700 1- Schober Kilian joint author ZORA 700 1- vom Berg Johannes joint author ZORA 700 1- Tresch Achim joint author ZORA 700 1- Grandl Martina joint author ZORA 700 1- Palle Pushpalatha joint author ZORA 700 1- Mair Florian joint author ZORA 700 1- Gerhard Markus joint author ZORA 700 1- Becher Burkhard joint author ZORA 700 1- Busch Dirk H. joint author ZORA 700 1- Buch Thorsten joint author ZORA 773 0- Scientific Reports 6:21377 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article openAccess