caa a22 4500 378849344 CHVBK 20180305123319.0 cr unu---uuuuu 161128e20030919xx s 000 0 eng 10.1515/CCLM.2003.202 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.202 Characteristics of Prolidase from the Erythrocytes of Normal Humans and Patients with Prolidase Deficiency and Their Mother [Elektronische Daten] [Kazuko Nakayama, Shiro Awata, Jianying Zhang, Hideto Kaba, Masanobu Manabe, Hiroyuki Kodama] Prolidases I and II were highly purified from human erythrocytes. The effects of various amino acids, MnCl2 and mercaptoethanol, on these two enzymes were investigated. Normal prolidase II was very labile in the absence of MnCl2 or mercaptoethanol. The activity of prolidase II was maintained at about 76% by preincubation with MnCl2; it was then activated up to 140% by treatment with mercaptoethanol for 60 minutes at 37 °C. Normal prolidases I and II showed the highest activity against glycylproline or methionylproline in the presence of MnCl2. The activity of prolidase I against glycylproline was enhanced strongly by glycine and MnCl2, but not activated in the absence of MnCl2. The activity of prolidase II against methionylproline was enhanced three-fold in the presence of glycine and MnCl2, but its activity against glycylproline was very low even in the presence of MnCl2. A stronger enhancement of this activity was found in normal erythrocytes, and a lower level of this activity was found in erythrocytes of patients treated with glycine, MnCl2 and mercaptoethanol compared to those treated with glycine and MnCl2. The activity of prolidase II against methionylproline in all erythrocytes, of normal humans and of patients, was strongly activated by the addition of glycine with MnCl2 but suppressed by the addition of mercaptoethanol. Clin Chem Lab Med 2003; 41(10):13231328 Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Nakayama Kazuko aut Awata Shiro aut Zhang Jianying aut Kaba Hideto aut Manabe Masanobu aut Kodama Hiroyuki aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/10(2003-09-19), 1323-1328 1434-6621 41:10<1323 2003 41 cclm https://doi.org/10.1515/CCLM.2003.202 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.202 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakayama Kazuko aut NATIONALLICENCE 700 1- Awata Shiro aut NATIONALLICENCE 700 1- Zhang Jianying aut NATIONALLICENCE 700 1- Kaba Hideto aut NATIONALLICENCE 700 1- Manabe Masanobu aut NATIONALLICENCE 700 1- Kodama Hiroyuki aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/10(2003-09-19), 1323-1328 1434-6621 41:10<1323 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter