caa a22 4500 378849417 CHVBK 20180305123319.0 cr unu---uuuuu 161128e20030919xx s 000 0 eng 10.1515/CCLM.2003.205 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.205 Differences between Genotyping and Phenotyping Methods for Assessing Apolipoprotein(a) Size Polymorphisms [Elektronische Daten] [Josep M. Simó, Jordi Camps, Silvia Martín, Juan Pedro-Botet, Natalia Ferré, Frederic Gómez, Jorge Joven] The aim of the present study was to analyze, on a double-blind basis, the relationships between the apolipoprotein(a) (apo(a)) gene and protein size polymorphisms in healthy volunteers (n = 99) and patients with premature myocardial infarction (n = 91). Apo(a) genotypes were determined by pulse-field electrophoresis and phenotypes were separated by sodium dodecyl sulfate-agarose gel electrophoresis. Results showed that phenotyping overestimated apo(a) size with respect to genotyping (mean (SD) = 3.7 (3.4) kringle units; p < 0.001) in subjects with a double-band genotype, although both measurements were highly correlated (r = 0.83; p < 0.001). We also observed that the protein band in subjects with a single-band phenotype was related more closely to the smallest allele than to the largest allele band. The correlation of plasma lipoprotein(a) (Lp(a)) concentration was stronger with the phenotype than with the genotype. We hypothesize that posttranslational modifications in the apo(a) molecule may be the most plausible explanation for the discrepancies observed. In conclusion, the present study highlights the dissimilarities between phenotyping and genotyping methods for the measurement of apo(a) size and suggests that laboratories should carefully consider these relationships and the transfer of results between such methodologies. Clin Chem Lab Med 2003; 41(10): 13401344 Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Simó Josep M. aut Camps Jordi aut Martín Silvia aut Pedro-Botet Juan aut Ferré Natalia aut Gómez Frederic aut Joven Jorge aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/10(2003-09-19), 1340-1344 1434-6621 41:10<1340 2003 41 cclm https://doi.org/10.1515/CCLM.2003.205 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.205 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Simó Josep M. aut NATIONALLICENCE 700 1- Camps Jordi aut NATIONALLICENCE 700 1- Martín Silvia aut NATIONALLICENCE 700 1- Pedro-Botet Juan aut NATIONALLICENCE 700 1- Ferré Natalia aut NATIONALLICENCE 700 1- Gómez Frederic aut NATIONALLICENCE 700 1- Joven Jorge aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/10(2003-09-19), 1340-1344 1434-6621 41:10<1340 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter