DNA Polymerase β Gene Expression: The Promoter Activator CREB-1 Is Upregulated in Chinese Hamster Ovary Cells by DNA Alkylating Agent-Induced Stress
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| 024 | 7 | 0 | |a 10.1515/BC.2003.003 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2003.003 | ||
| 245 | 0 | 0 | |a DNA Polymerase β Gene Expression: The Promoter Activator CREB-1 Is Upregulated in Chinese Hamster Ovary Cells by DNA Alkylating Agent-Induced Stress |h [Elektronische Daten] |c [F. He, X.-P. Yang, D.K. Srivastava, S.H. Wilson] |
| 520 | 3 | |a The DNA alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) upregulates the level of the base excision DNA repair enzyme DNA polymerase β (β-pol) in several mammalian cell types. Previous studies suggested that β-pol expression is upregulated via a transcriptional mechanism that requires: the specific cAMP response element (CRE) in the β-pol core promoter; a phosphorylated form of CREbinding protein-1 (CREB-1); and cellular protein kinase A activity. A large family of CRE-binding proteins, i.e., the ATF/CREB factors, has been identified in various cell types. This study further examines the role of CREbinding proteins in regulating β-pol expression through study of Chinese hamster ovary (CHO) cells. In CHO cell nuclear extract, CREB-1 and ATF-1 are the predominant CRE-binding protein family members recognizing the CRE in the β-pol core promoter. The concentration of CREB-1 increases strongly in CHO cells after exposure to MNNG. In contrast, the level of ATF-1 does not change after MNNG treatment. Recombinant expression of CREB-1 in CHO cells is sufficient to increase expression of the endogenous β-pol gene, even in the absence of MNNG exposure. These results indicate that β-pol gene expression in CHO cells can be upregulated by CREB-1 and that the activation of β-pol gene expression in response to DNA alkylating agent exposure involves a strong increase in the level of CREB-1. | |
| 540 | |a Copyright © 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 700 | 1 | |a He |D F. |4 aut | |
| 700 | 1 | |a Yang |D X.-P |4 aut | |
| 700 | 1 | |a Srivastava |D D.K. |4 aut | |
| 700 | 1 | |a Wilson |D S.H. |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 384/1(2003-01-27), 19-23 |x 1431-6730 |q 384:1<19 |1 2003 |2 384 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2003.003 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2003.003 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a He |D F. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D X.-P |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Srivastava |D D.K. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wilson |D S.H. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 384/1(2003-01-27), 19-23 |x 1431-6730 |q 384:1<19 |1 2003 |2 384 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||