caa a22 4500 378849549 CHVBK 20180305123320.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/BC.2003.003 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.003 DNA Polymerase β Gene Expression: The Promoter Activator CREB-1 Is Upregulated in Chinese Hamster Ovary Cells by DNA Alkylating Agent-Induced Stress [Elektronische Daten] [F. He, X.-P. Yang, D.K. Srivastava, S.H. Wilson] The DNA alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) upregulates the level of the base excision DNA repair enzyme DNA polymerase β (β-pol) in several mammalian cell types. Previous studies suggested that β-pol expression is upregulated via a transcriptional mechanism that requires: the specific cAMP response element (CRE) in the β-pol core promoter; a phosphorylated form of CREbinding protein-1 (CREB-1); and cellular protein kinase A activity. A large family of CRE-binding proteins, i.e., the ATF/CREB factors, has been identified in various cell types. This study further examines the role of CREbinding proteins in regulating β-pol expression through study of Chinese hamster ovary (CHO) cells. In CHO cell nuclear extract, CREB-1 and ATF-1 are the predominant CRE-binding protein family members recognizing the CRE in the β-pol core promoter. The concentration of CREB-1 increases strongly in CHO cells after exposure to MNNG. In contrast, the level of ATF-1 does not change after MNNG treatment. Recombinant expression of CREB-1 in CHO cells is sufficient to increase expression of the endogenous β-pol gene, even in the absence of MNNG exposure. These results indicate that β-pol gene expression in CHO cells can be upregulated by CREB-1 and that the activation of β-pol gene expression in response to DNA alkylating agent exposure involves a strong increase in the level of CREB-1. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence He F. aut Yang X.-P aut Srivastava D.K. aut Wilson S.H. aut Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 19-23 1431-6730 384:1<19 2003 384 bchm https://doi.org/10.1515/BC.2003.003 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.003 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He F. aut NATIONALLICENCE 700 1- Yang X.-P aut NATIONALLICENCE 700 1- Srivastava D.K. aut NATIONALLICENCE 700 1- Wilson S.H. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 19-23 1431-6730 384:1<19 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter