caa a22 4500 378849565 CHVBK 20180305123320.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/BC.2003.008 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.008 MSP-1 Malaria Pseudopeptide Analogs:Biological and Immunological Significance andThree-Dimensional Structure [Elektronische Daten] [J.M. Lozano, M.P. Alba, M. Vanegas, Y. Silva, J.L. Torres-Castellanos, M.E. Patarroyo] Merozoite Surface Protein-1 (MSP-1) has been considered as a malaria vaccine candidate. It is processed during the Plasmodium falciparum invasion process of red blood cells (RBCs). A conserved MSP-1 C-terminal peptide was identified as a high-activity erythrocyte binding peptide (HAEBP) termed 1585. Since conserved HAEBPs are neither antigenic nor immunogenic we decided to assess the significance of a single peptide bond replacement in 1585. Thus, two pseudopeptides were obtained by introducing a ψ[CH2-NH] reduced amide isoster into the 1585 critical binding motif. The pseudopeptides bound to different HLA-DR alleles, suggesting that backbone modifications affect MHC-II binding patterns. Pseudopeptide antibodies inhibit in vitro parasite RBC invasion by recognizing MSP-1. Each pseudopeptide-induced antibody shows distinct recognition patterns. 1H-NMR studies demonstrated that isoster bonds modulate the pseudopeptides structure and thus their immunological properties, therefore representing a possible subunit malaria vaccine component. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Lozano J.M. aut Alba M.P. aut Vanegas M. aut Silva Y. aut Torres-Castellanos J.L. aut Patarroyo M.E. aut Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 71-82 1431-6730 384:1<71 2003 384 bchm https://doi.org/10.1515/BC.2003.008 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lozano J.M. aut NATIONALLICENCE 700 1- Alba M.P. aut NATIONALLICENCE 700 1- Vanegas M. aut NATIONALLICENCE 700 1- Silva Y. aut NATIONALLICENCE 700 1- Torres-Castellanos J.L. aut NATIONALLICENCE 700 1- Patarroyo M.E. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 71-82 1431-6730 384:1<71 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter