Purification of Drosophila melanogasterUltraspiracle Protein and Analysis of Its A/BRegion-Dependent Dimerization Behavior invitro
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| 024 | 7 | 0 | |a 10.1515/BC.2003.007 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2003.007 | ||
| 245 | 0 | 0 | |a Purification of Drosophila melanogasterUltraspiracle Protein and Analysis of Its A/BRegion-Dependent Dimerization Behavior invitro |h [Elektronische Daten] |c [G. Rymarczyk, I. Grad, A. Rusek, K. Oswiecimska-Rusin, A. Niedziela-Majka, M. Kochman, A. Ozyhar] |
| 520 | 3 | |a Two members of the nuclear receptor superfamily, EcR (ecdysteroid receptor protein) and Usp (Ultraspiracle), heterodimerize to form a functional receptor for the steroid hormone 20-hydroxyecdysone and thus enable it to coordinate morphogenetic events during insect metamorphosis. Nterminally Histagged Usp was overexpressed in E. coli cells as a nontruncated protein and purified to homogeneity in two chromatographic steps. It was demonstrated that the recombinant receptor specifically binds the ecdysone response element of the hsp27 gene promoter (hsp27EcRE). Moreover, ahighly synergistically formed heterodimeric complex with the DNA-binding domain of EcR was observed on hsp27EcRE, but not on the native Usp response element from the chorion s15 gene promoter. Recombinant Usp forms homodimers and homotetramers in the absence of DNA, as judged from gel filtration and chemical crosslinking experiments. Truncation of its N-terminal A/B region changes molecular characteristics of Usp, considerably weakening its oligomerization potential under the same experimental conditions. This contrasts with the results obtained previously for the similarly truncated RXR - a vertebrate homolog of Usp. | |
| 540 | |a Copyright © 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 700 | 1 | |a Rymarczyk |D G. |4 aut | |
| 700 | 1 | |a Grad |D I. |4 aut | |
| 700 | 1 | |a Rusek |D A. |4 aut | |
| 700 | 1 | |a Oswiecimska-Rusin |D K. |4 aut | |
| 700 | 1 | |a Niedziela-Majka |D A. |4 aut | |
| 700 | 1 | |a Kochman |D M. |4 aut | |
| 700 | 1 | |a Ozyhar |D A. |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 384/1(2003-01-27), 59-69 |x 1431-6730 |q 384:1<59 |1 2003 |2 384 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2003.007 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2003.007 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rymarczyk |D G. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grad |D I. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rusek |D A. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oswiecimska-Rusin |D K. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Niedziela-Majka |D A. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kochman |D M. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ozyhar |D A. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 384/1(2003-01-27), 59-69 |x 1431-6730 |q 384:1<59 |1 2003 |2 384 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||