caa a22 4500 37884959X CHVBK 20180305123320.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/BC.2003.019 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.019 ‘Piggy-Back' Transport of Xenopus Hyaluronan Synthase (XHAS1) via the Secretory Pathway to the Plasma Membrane [Elektronische Daten] [J. Müllegger, A. Rustom, G. Kreil, H.-H. Gerdes, G. Lepperdinger] Hyaluronan is the sole glycosaminoglycan whose biosynthesis takes place directly at the plasma membrane. The mechanism by which hyaluronan synthase (HAS) becomes inserted there, as well as the question of how the enzyme discriminates between particular membrane species in polarized cells, are largely unknown. In vitro translation of HAS suggested that the nascent protein becomes stabilized in the presence of microsomal membranes, but would not insert spontaneously into membranes after being translated in the absence of those. We therefore monitored the membrane attachment of enzymatically active fusion proteins consisting of Xenopus HAS1 and green fluorescent protein shortly after de novo synthesis in Vero cells. Our data strongly suggest that HAS proteins are directly translated on the ER membrane without exhibiting an N-terminal signal sequence. From there the inactive protein is transferred to the plasma membrane via the secretory pathway. For unknown reasons, HAS inserted into membranes other than the plasma membrane remains inactive. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Müllegger J. aut Rustom A. aut Kreil G. aut Gerdes H.-H aut Lepperdinger G. aut Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 175-182 1431-6730 384:1<175 2003 384 bchm https://doi.org/10.1515/BC.2003.019 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.019 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Müllegger J. aut NATIONALLICENCE 700 1- Rustom A. aut NATIONALLICENCE 700 1- Kreil G. aut NATIONALLICENCE 700 1- Gerdes H.-H aut NATIONALLICENCE 700 1- Lepperdinger G. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 175-182 1431-6730 384:1<175 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter