caa a22 4500 378849654 CHVBK 20180305123320.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/BC.2003.015 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.015 Thiol Antioxidant Reversal of Pyrrolidine Dithiocarbamate-Induced Reciprocal Regulation of AP-1 and NF-κB [Elektronische Daten] [C.H. Kim, J.H. Kim, J. Lee, C.Y. Hsu, Y.S. Ahn] Pyrrolidine dithiocarbamate (PDTC) has been shown to have unique reciprocal activities in activating AP-1 and inhibiting NF-κB, two oxidative stresssensitive transcription factors. The opposing effects of PDTC on these two transcription factors have been attributed to its thiol antioxidant properties. In the present study, PDTC activation of AP-1, like its inhibition of NFκB, in bovine cerebral endothelial cells (BCECs) was zinc-dependent, consistent with the contention that PDTC acts as a zinc ionophore and the apparent reciprocal actions of PDTC are mediated by zinc. Unlike PDTC, other thiols and non-thiol antioxidants did not activate AP-1 on their own. Thiol, but not nonthiol, antioxidants reversed PDTC actions on AP-1 and NF-κB. PDTC reduced the intracellular glutathione content, and depletion of the cellular glutathione store by buthionine sulfoximine (BSO) further augmented PDTC actions on AP-1 and NF-κB. Nacetyl cysteine (NAC), a thiol antioxidant, reversed PDTC actions even after irreversible depletion of the cellular glutathione store by BSO. These findings together suggest that thiol antioxidant reversal of PDTC actions on AP-1 and NF-κB is independent of their established roles in scavenging oxygen free radicals or repleting the cellular glutathione content. The results in the present and earlier studies suggest that thiol antioxidants are likely to act as metal chelators that buffer zinc mediation of the reciprocal actions of PDTC on AP-1 and NF-κB. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Kim C.H. aut Kim J.H. aut Lee J. aut Hsu C.Y. aut Ahn Y.S. aut Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 143-150 1431-6730 384:1<143 2003 384 bchm https://doi.org/10.1515/BC.2003.015 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.015 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim C.H. aut NATIONALLICENCE 700 1- Kim J.H. aut NATIONALLICENCE 700 1- Lee J. aut NATIONALLICENCE 700 1- Hsu C.Y. aut NATIONALLICENCE 700 1- Ahn Y.S. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 143-150 1431-6730 384:1<143 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter