Conserved Repressive Regulation ofConnective Tissue Growth Factor/HypertrophicChondrocyte-Specific Gene 24 (ctgf/hcs24)Enabled by Different Elements and Factorsamong Vertebrate Species
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| 024 | 7 | 0 | |a 10.1515/BC.2003.001 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2003.001 | ||
| 245 | 0 | 0 | |a Conserved Repressive Regulation ofConnective Tissue Growth Factor/HypertrophicChondrocyte-Specific Gene 24 (ctgf/hcs24)Enabled by Different Elements and Factorsamong Vertebrate Species |h [Elektronische Daten] |c [Y. Mukudai, S. Kubota, M. Takigawa] |
| 520 | 3 | |a CTGF/Hcs24 is a multifunctional growth factor that potentiates the growth and differentiation of various cells. Our previous study revealed that the 3'-UTR of mammalian CTGF/Hcs24 mRNA contains a small segment that represses the gene expression in cis fashion. In this study, we isolated and characterized a chicken CTGF/Hcs24 cDNA clone. Chicken ctgf/hcs24 mRNA showed highly conserved homology in the ORF to that of mammalian species, whereas the homology in the 3'-UTR was relatively low. Northern blotting analysis revealed that chicken ctgf/hcs24 mRNA was expressed most strongly in cartilage, and also in brain, lung, heart, but faintly in liver. Thereafter we analyzed the functional potential of the 3'-UTR of ctgf/hcs24 cDNA to regulate its gene expression by reporter gene assay, and found that it repressed gene expression in cis fashion, specifically in avian cells, but not in mammalian cells. Conversely, the mammalian 3'-UTR showed less repressive activity in avian cells than in mammalian cells. Deletion analysis showed that a segment near the polyadenyl tail of the 3'-UTR of chicken ctgf/hcs24 played an important functional role, unlike in the mammalian species. Thus, we uncovered a novel mode of functional conservation of the ctgf/hcs24 3 UTR among vertebrate species mediated by different factors. | |
| 540 | |a Copyright © 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 700 | 1 | |a Mukudai |D Y. |4 aut | |
| 700 | 1 | |a Kubota |D S. |4 aut | |
| 700 | 1 | |a Takigawa |D M. |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 384/1(2003-01-27), 1-9 |x 1431-6730 |q 384:1<1 |1 2003 |2 384 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2003.001 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2003.001 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mukudai |D Y. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kubota |D S. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Takigawa |D M. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 384/1(2003-01-27), 1-9 |x 1431-6730 |q 384:1<1 |1 2003 |2 384 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||