caa a22 4500 378849689 CHVBK 20180305123320.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/BC.2003.001 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.001 Conserved Repressive Regulation ofConnective Tissue Growth Factor/HypertrophicChondrocyte-Specific Gene 24 (ctgf/hcs24)Enabled by Different Elements and Factorsamong Vertebrate Species [Elektronische Daten] [Y. Mukudai, S. Kubota, M. Takigawa] CTGF/Hcs24 is a multifunctional growth factor that potentiates the growth and differentiation of various cells. Our previous study revealed that the 3'-UTR of mammalian CTGF/Hcs24 mRNA contains a small segment that represses the gene expression in cis fashion. In this study, we isolated and characterized a chicken CTGF/Hcs24 cDNA clone. Chicken ctgf/hcs24 mRNA showed highly conserved homology in the ORF to that of mammalian species, whereas the homology in the 3'-UTR was relatively low. Northern blotting analysis revealed that chicken ctgf/hcs24 mRNA was expressed most strongly in cartilage, and also in brain, lung, heart, but faintly in liver. Thereafter we analyzed the functional potential of the 3'-UTR of ctgf/hcs24 cDNA to regulate its gene expression by reporter gene assay, and found that it repressed gene expression in cis fashion, specifically in avian cells, but not in mammalian cells. Conversely, the mammalian 3'-UTR showed less repressive activity in avian cells than in mammalian cells. Deletion analysis showed that a segment near the polyadenyl tail of the 3'-UTR of chicken ctgf/hcs24 played an important functional role, unlike in the mammalian species. Thus, we uncovered a novel mode of functional conservation of the ctgf/hcs24 3 UTR among vertebrate species mediated by different factors. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Mukudai Y. aut Kubota S. aut Takigawa M. aut Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 1-9 1431-6730 384:1<1 2003 384 bchm https://doi.org/10.1515/BC.2003.001 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.001 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mukudai Y. aut NATIONALLICENCE 700 1- Kubota S. aut NATIONALLICENCE 700 1- Takigawa M. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/1(2003-01-27), 1-9 1431-6730 384:1<1 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter