caa a22 4500 378852868 CHVBK 20180305123327.0 cr unu---uuuuu 161128e200308 xx s 000 0 eng 10.1515/pteridines.2003.14.3.17 doi (NATIONALLICENCE)gruyter-10.1515/pteridines.2003.14.3.17 The Intracellular Distribution of Folate Derivatives in Pea Leaves [Elektronische Daten] [Sherwin Y. Chan, Edwin A. Cossins] After hydrolysis of polyglutamate derivatives, leaf extracts of pea (Pisum sativum L.) seedlings were examined for individual folates using high performance liquid chromatography and microbiological assays employing Lactobacillus rhamnosus and Pediococcus acidilactici. 14-day seedlings contained 0.17+0.01 nmol folate mg'1 protein that was predominantly methylated and associated with the cytosolic fraction. Percoli gradient-purified mitochondria and chloroplasls contained 11,0±2.3% and 8.4±0.6% of cellular folate, respectively. Mitochondrial folates (0.47+0.11 nmol folate mg"' protein) were predominately (90%) formylatcd and unsubstituted. In contrast, chloroplasts contained a less concentrated (0.02±0.001 nmol folate mg"' protein) folate pool consisting of approximately 30% methylated folate. The total folate content of pea leaves increased by 40% when dark-grown (etiolated) 9-day seedlings were exposed to light for 48 hours. During this light treatment, the mitochondrial folate pool, on a per mg protein basis, increased 10-fold. This light treatment also changed the composition of the mitochondrial folate pool with a shift occurring from methylated to formylated and unsubstituted derivatives. These changes in the folates of greening tissues are discussed in relation to the metabolism of glycine and serine that accompanies photorespiration in leaf itssues. © 2013 by Walter de Gruyter GmbH & Co. folates nationallicence mitochondria nationallicence chloroplasts nationallicence greening nationallicence photorespiration nationallicence high performance liquid chromatography nationallicence Pisum sativum L nationallicence Chan Sherwin Y. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada aut Cossins Edwin A. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada aut Pteridines De Gruyter 14/3(2003-08), 17-26 0933-4807 14:3<17 2003 14 pteridines https://doi.org/10.1515/pteridines.2003.14.3.17 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/pteridines.2003.14.3.17 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chan Sherwin Y. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada aut NATIONALLICENCE 700 1- Cossins Edwin A. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada aut NATIONALLICENCE 773 0- Pteridines De Gruyter 14/3(2003-08), 17-26 0933-4807 14:3<17 2003 14 pteridines CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 378838768 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter