caa a22 4500 378853066 CHVBK 20180305123328.0 cr unu---uuuuu 161128e20030820xx s 000 0 eng 10.1515/BC.2003.137 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.137 Clinical Relevance of Matrix Metalloproteinase-13 Determined with a New Highly Specific and Sensitive ELISA in Ascitic Fluid of Advanced Ovarian Carcinoma Patients [Elektronische Daten] [B. Hantke, N. Harbeck, B. Schmalfeldt, I. Claes, O. Hiller, M.-O. Luther, A. Welk, W. Kuhn, M. Schmitt, H. Tschesche, B. Muehlenweg] Matrix metalloproteinases (MMPs) are involved in many physiological and pathophysiological processes, including tumor cell invasion and metastasis. For one member of this family, MMP-13 (collagenase-3), a new, highly specific ELISA with a sensitivity of 0.5 ng MMP-13/ml was established. The protein levels of MMP-13 in ascitic fluids of 30 patients with advanced ovarian cancer FIGO stage III (n=19) and IV (n=11) were measured with this ELISA. Using a cutoff value of 0.5 ng MMP-13/mg total protein, two patient subpopulations with short (median 16 months) and long (median 36 months) overall survival were identified. Together with other prognostic markers, determination of MMP-13 in ascitic fluid may help to identify patients at risk for early death and help to individualize adjuvant therapy. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Hantke B. aut Harbeck N. aut Schmalfeldt B. aut Claes I. aut Hiller O. aut Luther M.-O aut Welk A. aut Kuhn W. aut Schmitt M. aut Tschesche H. aut Muehlenweg B. aut Biological Chemistry Walter de Gruyter 384/8(2003-08-20), 1247-1251 1431-6730 384:8<1247 2003 384 bchm https://doi.org/10.1515/BC.2003.137 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.137 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hantke B. aut NATIONALLICENCE 700 1- Harbeck N. aut NATIONALLICENCE 700 1- Schmalfeldt B. aut NATIONALLICENCE 700 1- Claes I. aut NATIONALLICENCE 700 1- Hiller O. aut NATIONALLICENCE 700 1- Luther M.-O aut NATIONALLICENCE 700 1- Welk A. aut NATIONALLICENCE 700 1- Kuhn W. aut NATIONALLICENCE 700 1- Schmitt M. aut NATIONALLICENCE 700 1- Tschesche H. aut NATIONALLICENCE 700 1- Muehlenweg B. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/8(2003-08-20), 1247-1251 1431-6730 384:8<1247 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter