caa a22 4500 378853570 CHVBK 20180305123329.0 cr unu---uuuuu 161128e20031204xx s 000 0 eng 10.1515/CCLM.2003.246 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.246 Proteome Analysis of Diseased Joints from Mice Suffering from Collagen-Induced Arthritis [Elektronische Daten] [Peter Lorenz, Marcus Bantscheff, Saleh M. Ibrahim, Hans-Jürgen Thiesen, Michael O. Glocker] Strains of mice that are susceptible to autoimmunity have provided experimental models to analyze the molecular basis for the complex multifactorial inheritance of human autoimmune disease. In this study proteins associated with collagen-induced arthritis (CIA) in mice were experimentally identified using a global proteomics approach. Two-dimensional gels of proteins from inflamed and non-inflamed joints showed a distinguished protein profile visualizing about 530 Coomassie-stained protein spots in the pH 4-7 range. A total of 76 spots were identified by peptide mass fingerprinting with good confidence. They included proteins of cytoskeletal origin, chaperones, enzymes and also some signal transduction molecules. Comparison to gels from non-inflamed paws pointed to proteins that were differentially expressed between the control and diseased state. Ferritin light chain and antioxidant protein 2 were slightly more abundant, lymphoid enhancer binding factor 1 slightly, but significantly, less abundant in inflamed paws. Fourteen of the identified proteins were the products of genes that had increased transcript levels in the diseased state. However, on the protein level no significant differences were found in comparison to the controls. This study provides us with the framework for more detailed approaches to understanding the complex disease arthritis that go beyond global proteomics. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Lorenz Peter aut Bantscheff Marcus aut Ibrahim Saleh M. aut Thiesen Hans-Jürgen aut Glocker Michael O. aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1622-1632 1434-6621 41:12<1622 2003 41 cclm https://doi.org/10.1515/CCLM.2003.246 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.246 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lorenz Peter aut NATIONALLICENCE 700 1- Bantscheff Marcus aut NATIONALLICENCE 700 1- Ibrahim Saleh M. aut NATIONALLICENCE 700 1- Thiesen Hans-Jürgen aut NATIONALLICENCE 700 1- Glocker Michael O. aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1622-1632 1434-6621 41:12<1622 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter