caa a22 4500 378853589 CHVBK 20180305123329.0 cr unu---uuuuu 161128e20031204xx s 000 0 eng 10.1515/CCLM.2003.243 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.243 Determination of Nicotine and Its Major Metabolite Cotinine in Plasma or Serum by Gas Chromatography-Mass Spectrometry Using Ion-Trap Detection [Elektronische Daten] [Emmanuelle Cognard, Christian Staub] A specific method has been developed for the quantitative determination of nicotine and its major metabolite cotinine in plasma or serum of active and passive smokers. Deuterium-labelled nicotine and cotinine were used as internal standards. The amounts of nicotine and cotinine present in a sample of plasma or serum were extracted with a simple extraction procedure (liquid-liquid or solid-phase extraction). The extracts were analysed by gas chromatography coupled with mass spectrometry using ion-trap detection. The analysis was done in positive chemical ionisation with methanol as the liquid reagent. The method has been demonstrated to be linear up to 1000 μg/l. Limits of quantification for nicotine and cotinine are 10 and 5 μg/l, respectively with liquid-liquid extraction, and 1 μg/l for each of the compounds with solid-phase extraction. The present method has been applied to several real cases. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Cognard Emmanuelle aut Staub Christian aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1599-1607 1434-6621 41:12<1599 2003 41 cclm https://doi.org/10.1515/CCLM.2003.243 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.243 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cognard Emmanuelle aut NATIONALLICENCE 700 1- Staub Christian aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1599-1607 1434-6621 41:12<1599 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter