caa a22 4500 378853597 CHVBK 20180305123329.0 cr unu---uuuuu 161128e20031204xx s 000 0 eng 10.1515/CCLM.2003.238 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.238 Application of Gas Chromatography-Mass Spectrometry for Analysis of Isoprostanes: Their Role in Cardiovascular Disease [Elektronische Daten] [Edzard Schwedhelm, Rainer H. Böger] Cardiovascular disease (CVD) is the major cause of death in the Western hemisphere. Oxidative stress is involved in the pathophysiology of cancer, neurodegenerative conditions and CVD. Lipid peroxidation is one of the oxidative modifications possible in biological systems. The isoprostanes are derivatives of one specific lipid, i.e., arachidonic acid, after lipid peroxidation. Several isoprostanes have been identified in biological tissues and fluids, among them 8-iso prostaglandin F2α (8-iso-PGF2α, 8-epi-PGF2α, iPF2α-III, 15-F2t-IsoP) and its metabolite, 2,3-dinor-4,5-dihydro-8-iso-PGF2α. The isoprostanes are reliable in vivo markers of lipid peroxidation in humans: they are endogenously formed, characteristic in structure, ubiquitous in nature, stable in- and ex vivo and reliably quantitatable. In this Review, different analytical approaches will be discussed including immunologic, chromatographic and spectrometric techniques with the main emphasis on mass spectrometry. Analysis of isoprostanes applying radio immunoassay (RIA), enzyme immunoassay (ElA), high performance-liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-tandem MS), gas chromatography-mass spectrometry (GC-MS) and GC-tandem MS will be exemplified in the field of cardiovascular research. Results from several clinical studies are included indicating the validity of isoprostanes as surrogate parameters of oxidative stress in cardiovascular disease. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Schwedhelm Edzard aut Böger Rainer H. aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1552-1561 1434-6621 41:12<1552 2003 41 cclm https://doi.org/10.1515/CCLM.2003.238 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.238 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schwedhelm Edzard aut NATIONALLICENCE 700 1- Böger Rainer H. aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1552-1561 1434-6621 41:12<1552 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter