Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method
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| 024 | 7 | 0 | |a 10.1515/CCLM.2003.247 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.247 | ||
| 245 | 0 | 0 | |a Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method |h [Elektronische Daten] |c [Debora Tagliacozzi, Alessia F. Mozzi, Bruno Casetta, Pierfrancesco Bertucci, Sergio Bernardini, Carmine Di Ilio, Andrea Urbani, Giorgio Federici] |
| 520 | 3 | |a Bile acids play a pivotal role in the metabolism of cholesterol and lipids. Their blood concentrations are important prognostic and diagnostic indicators of hepatobiliary and intestinal dysfunction. This class of molecules comprises a heterogeneous group of compounds with a common cholesterol scaffold. Recently, the introduction of liquid chromatography coupled to tandem mass spectrometry methods has revealed an innovative path in the quantisation of specific bile acids in biological specimens. A robust and sensitive method has been developed based on high performance liquid chromatography separation coupled to an electrospray triple-quadrupole mass spectrometer. Human plasma samples were analysed on a C18 reverse-phase column. The elution profiles were monitored in multiple reaction-monitoring mode, quantifying and identifying each analyte by its own unique precursor to product patterns. A linear correlation over a broad range of bile acid concentrations (0.1-100 μM) was observed. The average recovery period for all of the analysed bile acids was 98±3%. Intra-day and inter-day precision averages were 2% and 5.4%, respectively. The determination was achieved within a single chromatographic run for all unconjugated, glycine-and taurine-conjugated isomeric forms of bile acids. As a proof of principle this method has been validated on a small subset of cholestatic patients (n = 7) and compared to appropriate clinical controls (n = 10). Based upon our encouraging experimental results, the described HPLC separation coupled to tandem mass spectrometry method for the analysis of bile acids in biological samples is deemed a robust and accurate procedure. Consequently, we propose this technique as a suitable candidate method for the identification and quantitation of bile acids in routine analysis. | |
| 540 | |a Copyright (c) 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 700 | 1 | |a Tagliacozzi |D Debora |4 aut | |
| 700 | 1 | |a Mozzi |D Alessia F. |4 aut | |
| 700 | 1 | |a Casetta |D Bruno |4 aut | |
| 700 | 1 | |a Bertucci |D Pierfrancesco |4 aut | |
| 700 | 1 | |a Bernardini |D Sergio |4 aut | |
| 700 | 1 | |a Ilio |D Carmine Di |4 aut | |
| 700 | 1 | |a Urbani |D Andrea |4 aut | |
| 700 | 1 | |a Federici |D Giorgio |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/12(2003-12-04), 1633-1641 |x 1434-6621 |q 41:12<1633 |1 2003 |2 41 |o cclm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/CCLM.2003.247 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2003.247 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tagliacozzi |D Debora |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mozzi |D Alessia F. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Casetta |D Bruno |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bertucci |D Pierfrancesco |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bernardini |D Sergio |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ilio |D Carmine Di |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Urbani |D Andrea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Federici |D Giorgio |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/12(2003-12-04), 1633-1641 |x 1434-6621 |q 41:12<1633 |1 2003 |2 41 |o cclm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||