caa a22 4500 378853600 CHVBK 20180305123329.0 cr unu---uuuuu 161128e20031204xx s 000 0 eng 10.1515/CCLM.2003.247 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.247 Quantitative Analysis of Bile Acids in Human Plasma by Liquid Chromatography-Electrospray Tandem Mass Spectrometry: A Simple and Rapid One-Step Method [Elektronische Daten] [Debora Tagliacozzi, Alessia F. Mozzi, Bruno Casetta, Pierfrancesco Bertucci, Sergio Bernardini, Carmine Di Ilio, Andrea Urbani, Giorgio Federici] Bile acids play a pivotal role in the metabolism of cholesterol and lipids. Their blood concentrations are important prognostic and diagnostic indicators of hepatobiliary and intestinal dysfunction. This class of molecules comprises a heterogeneous group of compounds with a common cholesterol scaffold. Recently, the introduction of liquid chromatography coupled to tandem mass spectrometry methods has revealed an innovative path in the quantisation of specific bile acids in biological specimens. A robust and sensitive method has been developed based on high performance liquid chromatography separation coupled to an electrospray triple-quadrupole mass spectrometer. Human plasma samples were analysed on a C18 reverse-phase column. The elution profiles were monitored in multiple reaction-monitoring mode, quantifying and identifying each analyte by its own unique precursor to product patterns. A linear correlation over a broad range of bile acid concentrations (0.1-100 μM) was observed. The average recovery period for all of the analysed bile acids was 98±3%. Intra-day and inter-day precision averages were 2% and 5.4%, respectively. The determination was achieved within a single chromatographic run for all unconjugated, glycine-and taurine-conjugated isomeric forms of bile acids. As a proof of principle this method has been validated on a small subset of cholestatic patients (n = 7) and compared to appropriate clinical controls (n = 10). Based upon our encouraging experimental results, the described HPLC separation coupled to tandem mass spectrometry method for the analysis of bile acids in biological samples is deemed a robust and accurate procedure. Consequently, we propose this technique as a suitable candidate method for the identification and quantitation of bile acids in routine analysis. Copyright (c) 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Tagliacozzi Debora aut Mozzi Alessia F. aut Casetta Bruno aut Bertucci Pierfrancesco aut Bernardini Sergio aut Ilio Carmine Di aut Urbani Andrea aut Federici Giorgio aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1633-1641 1434-6621 41:12<1633 2003 41 cclm https://doi.org/10.1515/CCLM.2003.247 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.247 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tagliacozzi Debora aut NATIONALLICENCE 700 1- Mozzi Alessia F. aut NATIONALLICENCE 700 1- Casetta Bruno aut NATIONALLICENCE 700 1- Bertucci Pierfrancesco aut NATIONALLICENCE 700 1- Bernardini Sergio aut NATIONALLICENCE 700 1- Ilio Carmine Di aut NATIONALLICENCE 700 1- Urbani Andrea aut NATIONALLICENCE 700 1- Federici Giorgio aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1633-1641 1434-6621 41:12<1633 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter