Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids
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| 024 | 7 | 0 | |a 10.1515/CCLM.2003.241 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.241 | ||
| 245 | 0 | 0 | |a Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids |h [Elektronische Daten] |c [Peter Kleinert, Thomas Kuster, Silke Durka, Diana Ballhausen, Nils U. Bosshard, Beat Steinmann, Edgar Hänseler, Jaak Jaeken, Claus W. Heizmann, Heinz Troxler] |
| 520 | 3 | |a In this study, we present a versatile new procedure for the analysis of transferrin and its isoforms isolated from human body fluids such as serum, plasma, and cerebrospinal fluid. This method is based on a three-step procedure: (i) isolation of transferrins using anion-exchange chromatography with UV detection; (ii) concentration of the transferrin fraction; (iii) detection of the transferrins with liquid chromatography-electrospray mass spectrometry. Pre-analytical sample procedures can be omitted and no immunoaffinity columns or transferrin-specific immunoassays were used. Anticoagulants such as heparin, EDTA, citrate, and oxalate do not interfere with our analysis. According to their respective molecular masses, up to ten different isoforms of transferrin could be identified in a serum sample from a patient with a congenital disorder of glycosylation type Ia (CDG-Ia). The method was successfully applied to different pathological samples from patients with CDG-Ia, CDG-Ib, CDG-Ic, CDG-Ie, CDG-If, and CDG-IIa. Additionally, samples from alcohol consumers that were found with turbidimetric immunoassay to contain increased levels of carbohydrate-deficient transferrin were analyzed. | |
| 540 | |a Copyright © 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 700 | 1 | |a Kleinert |D Peter |4 aut | |
| 700 | 1 | |a Kuster |D Thomas |4 aut | |
| 700 | 1 | |a Durka |D Silke |4 aut | |
| 700 | 1 | |a Ballhausen |D Diana |4 aut | |
| 700 | 1 | |a Bosshard |D Nils U. |4 aut | |
| 700 | 1 | |a Steinmann |D Beat |4 aut | |
| 700 | 1 | |a Hänseler |D Edgar |4 aut | |
| 700 | 1 | |a Jaeken |D Jaak |4 aut | |
| 700 | 1 | |a Heizmann |D Claus W. |4 aut | |
| 700 | 1 | |a Troxler |D Heinz |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/12(2003-12-04), 1580-1588 |x 1434-6621 |q 41:12<1580 |1 2003 |2 41 |o cclm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/CCLM.2003.241 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2003.241 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kleinert |D Peter |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kuster |D Thomas |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Durka |D Silke |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ballhausen |D Diana |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bosshard |D Nils U. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Steinmann |D Beat |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hänseler |D Edgar |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jaeken |D Jaak |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Heizmann |D Claus W. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Troxler |D Heinz |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/12(2003-12-04), 1580-1588 |x 1434-6621 |q 41:12<1580 |1 2003 |2 41 |o cclm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||