caa a22 4500 378853627 CHVBK 20180305123329.0 cr unu---uuuuu 161128e20031204xx s 000 0 eng 10.1515/CCLM.2003.241 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.241 Mass Spectrometric Analysis of Human Transferrin in Different Body Fluids [Elektronische Daten] [Peter Kleinert, Thomas Kuster, Silke Durka, Diana Ballhausen, Nils U. Bosshard, Beat Steinmann, Edgar Hänseler, Jaak Jaeken, Claus W. Heizmann, Heinz Troxler] In this study, we present a versatile new procedure for the analysis of transferrin and its isoforms isolated from human body fluids such as serum, plasma, and cerebrospinal fluid. This method is based on a three-step procedure: (i) isolation of transferrins using anion-exchange chromatography with UV detection; (ii) concentration of the transferrin fraction; (iii) detection of the transferrins with liquid chromatography-electrospray mass spectrometry. Pre-analytical sample procedures can be omitted and no immunoaffinity columns or transferrin-specific immunoassays were used. Anticoagulants such as heparin, EDTA, citrate, and oxalate do not interfere with our analysis. According to their respective molecular masses, up to ten different isoforms of transferrin could be identified in a serum sample from a patient with a congenital disorder of glycosylation type Ia (CDG-Ia). The method was successfully applied to different pathological samples from patients with CDG-Ia, CDG-Ib, CDG-Ic, CDG-Ie, CDG-If, and CDG-IIa. Additionally, samples from alcohol consumers that were found with turbidimetric immunoassay to contain increased levels of carbohydrate-deficient transferrin were analyzed. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Kleinert Peter aut Kuster Thomas aut Durka Silke aut Ballhausen Diana aut Bosshard Nils U. aut Steinmann Beat aut Hänseler Edgar aut Jaeken Jaak aut Heizmann Claus W. aut Troxler Heinz aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1580-1588 1434-6621 41:12<1580 2003 41 cclm https://doi.org/10.1515/CCLM.2003.241 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.241 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kleinert Peter aut NATIONALLICENCE 700 1- Kuster Thomas aut NATIONALLICENCE 700 1- Durka Silke aut NATIONALLICENCE 700 1- Ballhausen Diana aut NATIONALLICENCE 700 1- Bosshard Nils U. aut NATIONALLICENCE 700 1- Steinmann Beat aut NATIONALLICENCE 700 1- Hänseler Edgar aut NATIONALLICENCE 700 1- Jaeken Jaak aut NATIONALLICENCE 700 1- Heizmann Claus W. aut NATIONALLICENCE 700 1- Troxler Heinz aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/12(2003-12-04), 1580-1588 1434-6621 41:12<1580 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter