caa a22 4500 378855956 CHVBK 20180305123335.0 cr unu---uuuuu 161128e20031225xx s 000 0 eng 10.1515/BC.2003.173 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.173 Effects of Iron Limitation on the Respiratory Chain and the Membrane Cytochrome Pattern of the Euryarchaeon Halobacterium salinarum [Elektronische Daten] [D. Hubmacher, B. F. Matzanke, S. Anemüller] The effects of iron limitation on the electron transport chain of the extremely halophilic Euryarchaeon Halobacterium salinarum were analyzed. When iron was growth-limiting, the respiratory rates as well as the inhibition pattern of the membranes were significantly different from membranes of iron replete cells. Changes in the availability of iron cause the formation of different respiratory pathways including different entry sites for electrons, different terminal oxidases of the respiratory chain, and drastic changes of the cytochrome composition and of the relative amounts of cytochromes. Under iron-limiting conditions, mainly low-potential cytochromes were measured. EPR spectroscopic studies revealed that the amount of proteins containing iron-sulfur clusters is reduced in membranes under iron-limiting growth conditions. Taken together, our results strongly suggest for the first time an important role of iron supply for the bioenergetics of an Archaeon. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Hubmacher D. aut Matzanke B. F. aut Anemüller S. aut Biological Chemistry Walter de Gruyter 384/12(2003-12-25), 1565-1573 1431-6730 384:12<1565 2003 384 bchm https://doi.org/10.1515/BC.2003.173 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.173 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hubmacher D. aut NATIONALLICENCE 700 1- Matzanke B. F. aut NATIONALLICENCE 700 1- Anemüller S. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/12(2003-12-25), 1565-1573 1431-6730 384:12<1565 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter