The Binding of Haptoglobin to Apolipoprotein AI: Influence of Hemoglobin and Concanavalin A
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| 024 | 7 | 0 | |a 10.1515/BC.2003.176 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/BC.2003.176 | ||
| 245 | 0 | 4 | |a The Binding of Haptoglobin to Apolipoprotein AI: Influence of Hemoglobin and Concanavalin A |h [Elektronische Daten] |c [M. S. Spagnuolo, L. Cigliano, P. Abrescia] |
| 520 | 3 | |a Haptoglobin (Hp) can be purified by affinity chromatography using hemoglobin (Hb)-linked Sepharose. Elution with 8 M urea is generally performed, resulting in heavy contamination of the Hp preparation by apolipoprotein AI (ApoAI), and partial loss of Hb binding activity. Hp, separated from ApoAI, was recovered by elution with glycine-HCl at pH 3. Complexes of the isolated protein with Hb or ApoAI were detected by enzyme-linked immunosorbent assay (ELISA). Competition between the two ligands in their interaction with Hp was observed. Concanavalin A (ConA), which binds the Hp carbohydrate chains, did not influence Hp binding to ApoAI. These results suggest that changes in the plasma levels of ApoAI or Hb affect the Hp role in regulating the reverse transport of cholesterol or preventing Hb-dependent oxidative damage. | |
| 540 | |a Copyright © 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Biochemistry |2 nationallicence | |
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a Cellular biology |2 nationallicence | |
| 700 | 1 | |a Spagnuolo |D M. S. |4 aut | |
| 700 | 1 | |a Cigliano |D L. |4 aut | |
| 700 | 1 | |a Abrescia |D P. |4 aut | |
| 773 | 0 | |t Biological Chemistry |d Walter de Gruyter |g 384/12(2003-12-25), 1593-1596 |x 1431-6730 |q 384:12<1593 |1 2003 |2 384 |o bchm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/BC.2003.176 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/BC.2003.176 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Spagnuolo |D M. S. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cigliano |D L. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abrescia |D P. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biological Chemistry |d Walter de Gruyter |g 384/12(2003-12-25), 1593-1596 |x 1431-6730 |q 384:12<1593 |1 2003 |2 384 |o bchm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
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