caa a22 4500 378858890 CHVBK 20180305123341.0 cr unu---uuuuu 161128e20031107xx s 000 0 eng 10.1515/BC.2003.160 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.160 Immunogenicity and Protectivity of Plasmodium falciparum EBA-175 Peptide and Its Analog Is Associated with α-Helical Region Shortening and Displacement [Elektronische Daten] [A. Bermúdez, G. Cifuentes, F. Guzmán, L. M. Salazar, M. E. Patarroyo] EBA-175 protein is used as a ligand in the binding of P. falciparum to red blood cells (RBCs). Evidence shows that the conserved peptide 1779 from this protein (with high red blood cell binding ability and known critical erythrocyte binding residues) plays an important role in the invasion process. This peptide is neither immunogenic nor protective; analogs having critical residues replaced by amino acids with similar volume or mass but different polarity were synthesized and inoculated into Aotus monkeys, and elicited different immunogenic and protective responses. Nuclear Magnetic Resonance (1H-NMR) studies revealed that peptide analog 21696 (non-immunogenic and non-protective) presents a large helical fragment, that the peptide 14012 (immunogenic and non-protective) helical fragment is smaller, while the peptide 22812 (immunogenic and protective) α-helix is shorter in a different region and possesses greater flexibility at its N-terminus. The presence of methionine residues could affect the structural stability of peptide 22812 and ultimately its immunological response. Our results suggest a new strategy for designing a new malaria multi-component subunit-based vaccine. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Bermúdez A. aut Cifuentes G. aut Guzmán F. aut Salazar L. M. aut Patarroyo M. E. aut Biological Chemistry Walter de Gruyter 384/10-11(2003-11-07), 1443-1450 1431-6730 384:10-11<1443 2003 384 bchm https://doi.org/10.1515/BC.2003.160 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.160 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bermúdez A. aut NATIONALLICENCE 700 1- Cifuentes G. aut NATIONALLICENCE 700 1- Guzmán F. aut NATIONALLICENCE 700 1- Salazar L. M. aut NATIONALLICENCE 700 1- Patarroyo M. E. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/10-11(2003-11-07), 1443-1450 1431-6730 384:10-11<1443 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter