caa a22 4500 378859048 CHVBK 20180305123342.0 cr unu---uuuuu 161128e20031107xx s 000 0 eng 10.1515/BC.2003.169 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.169 Mast Cell Cathepsins C and S Control Levels of Carboxypeptidase A and the Chymase, Mouse Mast Cell Protease 5 [Elektronische Daten] [F. Henningsson, P. Wolters, H. A. Chapman, G. H. Caughey, G. Pejler] Carboxypeptidase A (CPA) is a metalloprotease, residing in the mast cell secretory granules together with chymases and tryptases. Little information is available with respect to the mechanisms that maintain or regulate the levels of stored proteases in the mast cell secretory granules. In this study we examined whether cathepsins C and S may be involved in the control of the levels of mast cell proteases. Mast cells cultured from bone marrow of cathepsin C or Snull mice expressed higher levels of CPA protein and activity than cells from wild-type mice. Similar increases in protein were observed for the mouse chymase, mast cell protease-5 (mMCP-5), but not for the tryptase, mMCP-6. Steady-state levels of CPA and mMCP-5 mRNA were similar in wildtype and cathepsin C-null mast cells, indicating that posttranscriptional mechanisms explain the observed cathepsin C-dependence of CPA and mMCP-5 expression. The present study thus indicates novel roles for cathepsins C and S in regulating the levels of stored proteases in the mast cell secretory granules. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Henningsson F. aut Wolters P. aut Chapman H. A. aut Caughey G. H. aut Pejler G. aut Biological Chemistry Walter de Gruyter 384/10-11(2003-11-07), 1527-1531 1431-6730 384:10-11<1527 2003 384 bchm https://doi.org/10.1515/BC.2003.169 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.169 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henningsson F. aut NATIONALLICENCE 700 1- Wolters P. aut NATIONALLICENCE 700 1- Chapman H. A. aut NATIONALLICENCE 700 1- Caughey G. H. aut NATIONALLICENCE 700 1- Pejler G. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/10-11(2003-11-07), 1527-1531 1431-6730 384:10-11<1527 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter