caa a22 4500 378861395 CHVBK 20180305123347.0 cr unu---uuuuu 161128e20030101xx s 000 0 eng 10.1351/pac200375112451 doi (NATIONALLICENCE)gruyter-10.1351/pac200375112451 Biomonitoring: Integration of biological end-points into chemical monitoring [Elektronische Daten] [M. Seifert, L. Wen, M. Alberti, U. Kausch, B. Hock] Biomonitoring is currently performed at two levels, assessing exposure to pollutants and effects monitoring by bioassays. As an example for the first approach, vitellogenin (VTG) in male fish of Abramis brama as an endpoint for estrogen exposure is discussed. However, similar changes of VTG or VTG-like proteins in the hemolymph of mussels could not be detected. Enzyme-linked receptor assays for monitoring estrogenic effects at the molecular level serve as an example for the second category. Applications of the enzyme-linked receptor assay (ELRA) developed in our laboratory are presented. Detection limits of 0.02 μg/l 17β-estradiol were recently achieved with the chemiluminescent format. Although effect monitoring provides information in terms of toxicity equivalents, it is not possible to relate the signals to specific pollutants and their concentrations. For this purpose, chemical analysis is required. New approaches are reported for the direct coupling of bioassays and chemical analysis. This concept is defined as bioresponse-linked instrumental analysis. It combines biomolecular recognition, initiating a biological effect, and chemical analysis. In addition to the classical bioanalytical approaches, new strategies in genomics and proteomics have been developed. This may lead to multimarker approaches opening this area to environmental analytics. © 2013 Walter de Gruyter GmbH, Berlin/Boston Seifert M. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut Wen L. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut Alberti M. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut Kausch U. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut Hock B. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut Pure and Applied Chemistry De Gruyter 75/11-12(2003-01-01), 2451-2459 0033-4545 75:11-12<2451 2003 75 pac https://doi.org/10.1351/pac200375112451 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1351/pac200375112451 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seifert M. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut NATIONALLICENCE 700 1- Wen L. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut NATIONALLICENCE 700 1- Alberti M. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut NATIONALLICENCE 700 1- Kausch U. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut NATIONALLICENCE 700 1- Hock B. Technische Universitaet Muenchen,Center of Life Sciences Weihenstephan, Chair of Cell Biology, Alte Akademie12, D-85350 Freising, Germany aut NATIONALLICENCE 773 0- Pure and Applied Chemistry De Gruyter 75/11-12(2003-01-01), 2451-2459 0033-4545 75:11-12<2451 2003 75 pac CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter