The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using CapillaryElectrophoresis
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| 024 | 7 | 0 | |a 10.1515/CCLM.2003.113 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.113 | ||
| 245 | 0 | 4 | |a The Analysis of Carbohydrate-Deficient Transferrin, Marker of Chronic Alcoholism, Using CapillaryElectrophoresis |h [Elektronische Daten] |c [Birgitte Wuyts, Joris R. Delanghe] |
| 520 | 3 | |a Carbohydrate-deficient transferrin (CDT) is currently considered to be the best available marker for the diagnosis of chronic alcoholism. A large variety of methods have been developed, demonstrating the need for standardisation. Commercially available anion-exchange chromatographic-based assays are easy to use and require no specialised, expensive instruments. However, these methods cannot identify genetic transferrin variants or the carbohydrate-deficient glycoprotein syndrome. In 1989, a capillary isoelectric focusing method was developed for quantitative measurement of CDT. Despite the optimal resolution, this method is not easily applied in a clinical routine environment due to the complexity of analysis. Capillary electrophoresis in a polymer network using coated capillaries allowed full resolution of the sialoforms of human transferrin. The drawbacks due to an expensive and time-consuming sample preparation were eliminated when a method in neat serum was developed. Capillary zone electrophoresis allowed full resolution of the transferrin isoforms with a high analytical performance in a short analysis time thanks to a strong electroosmotic flow. Genetic transferrin variants were easily detected, avoiding false-positive results. Also, using capillary zone electrophoresis, it was shown that CDT is a suitable marker of chronic alcohol abuse detection in transferrin CD (common/cathodal) variants. | |
| 540 | |a Copyright © 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 700 | 1 | |a Wuyts |D Birgitte |4 aut | |
| 700 | 1 | |a Delanghe |D Joris R. |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/6(2003-06-17), 739-746 |x 1434-6621 |q 41:6<739 |1 2003 |2 41 |o cclm | |
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| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2003.113 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wuyts |D Birgitte |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Delanghe |D Joris R. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/6(2003-06-17), 739-746 |x 1434-6621 |q 41:6<739 |1 2003 |2 41 |o cclm | ||
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