caa a22 4500 378867229 CHVBK 20180305123400.0 cr unu---uuuuu 161128e20030617xx s 000 0 eng 10.1515/CCLM.2003.112 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.112 Capillary Electrophoresis - A High Performance Analytical Separation Technique [Elektronische Daten] [Hermann Wätzig, Stefan Günter] Capillary electrophoresis (CE) is often one of the preferred techniques in pharmaceutical quality control and in clinical chemistry, particularly considering the high selectivity and lower costs compared to HPLC. The precision of CE is as good as in liquid chromatography (LC). The sample-throughput is high due to short analysis times. Efforts for sample pre-treatment are usually minor in CE. Urine and even blood plasma can be directly injected without further pre-treatment. After summarising the basic principles of CE, general strategies for method development are described to achieve selective, efficient, precise, fast, sensitive, and validated methods. Sample pre-treatment requirements are discussed. Standard buffer recipes, surfactants used in micellar electrokinetic chromatography (MEKC), chiral selectors, useful buffer additives, actions to deal with complex matrices, and aspects of validation have been collected. Other techniques that can be performed with CE instruments, such as capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), and capillary electrochromatography (CEC), are briefly discussed. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Wätzig Hermann aut Günter Stefan aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 724-738 1434-6621 41:6<724 2003 41 cclm https://doi.org/10.1515/CCLM.2003.112 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.112 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wätzig Hermann aut NATIONALLICENCE 700 1- Günter Stefan aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 724-738 1434-6621 41:6<724 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter