caa a22 4500 378867253 CHVBK 20180305123400.0 cr unu---uuuuu 161128e20030617xx s 000 0 eng 10.1515/CCLM.2003.118 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.118 Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions [Elektronische Daten] [Paola Luraschi, Elisabetta Dalla Dea, Carlo Franzini] Analytical conditions in a system for capillary zone electrophoresis (Beckman Paragon CZE 2000) were originally selected to allow serum protein separation into five discrete protein zones, corresponding to those of conventional clinical electrophoresis. To improve the system's performance, new analytical conditions have been made available. We compared the two sets of conditions ("new" = y; "old" = x) for possible variations of results caused by the change. One hundred thirteen serum samples, covering wide intervals of values, were assayed on two twin instruments working under the old and the new conditions; results were assessed statistically and graphically. Possible clinical significance of differences was checked by comparison with the biological variation-based quality specifications for bias. Statistically significant (y-x) differences were observed for the α1-, α2- and β-globulin zones; clinically significant differences were observed for all the zones, with the exception of the γ-globulin zone. Therefore, old/new regression equations were calculated, whose reliability was assured by the wide interval of values, by the large sample size, and by the low dispersion of single values around the mean concordance estimates. Such equations may be used to convert "old" into "new" reference values, and for the intercomparison of patient results obtained under different analytical conditions. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Luraschi Paola aut Dea Elisabetta Dalla aut Franzini Carlo aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 782-786 1434-6621 41:6<782 2003 41 cclm https://doi.org/10.1515/CCLM.2003.118 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.118 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luraschi Paola aut NATIONALLICENCE 700 1- Dea Elisabetta Dalla aut NATIONALLICENCE 700 1- Franzini Carlo aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 782-786 1434-6621 41:6<782 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter