caa a22 4500 37886727X CHVBK 20180305123400.0 cr unu---uuuuu 161128e20030617xx s 000 0 eng 10.1515/CCLM.2003.119 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.119 Type-Specific Detection of Human Papillomaviruses in a Routine Laboratory Setting - Improved Sensitivity and Specificity of PCR and Sequence Analysis Compared to Direct Hybridisation [Elektronische Daten] [Siegfried Kösel, Siegfried Burggraf, Jens Mommsen, Werner Engelhardt, Bernhard Olgemöller] Human papillomaviruses (HPV) are known to cause cervical dysplasia and cervical carcinoma. We used a 3-step PCR protocol that allows rapid type-specific HPV testing in a routine laboratory setting: HPV-16-positive samples were determined using a specific Light-Cycler PCR; HPV-16-negative samples were amplified by nested PCR and typed by sequence analysis. During a period of 7 months, 1275 PCR-based HPV tests were performed. Of the 1275 samples, 829 samples tested negative for HPV and 446 tested positive, including 124 positives found in the initial HPV-16-specific Light-Cycler assay. Sequence analysis of 132 samples detected 18 HPV types that are not included in the widely used Hybrid Capture II assay. For comparison, the first 100 cervical specimens were tested in parallel using PCR and direct hybridisation (Hybrid Capture II assay). PCR detected HPV DNA in 23 samples that tested negative in the Hybrid Capture assay. Four out of 37 samples that tested positive for HPV in the Hybrid Capture test may be false positives, because sequence analysis detected HPV types not included in the probe mixtures. As rare and novel HPV types may also confer an oncogenic risk, highly sensitive and specific PCR assays will help in understanding cervical HPV infection and cervical cancer of unknown causes. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Kösel Siegfried aut Burggraf Siegfried aut Mommsen Jens aut Engelhardt Werner aut Olgemöller Bernhard aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 787-791 1434-6621 41:6<787 2003 41 cclm https://doi.org/10.1515/CCLM.2003.119 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.119 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kösel Siegfried aut NATIONALLICENCE 700 1- Burggraf Siegfried aut NATIONALLICENCE 700 1- Mommsen Jens aut NATIONALLICENCE 700 1- Engelhardt Werner aut NATIONALLICENCE 700 1- Olgemöller Bernhard aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 787-791 1434-6621 41:6<787 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter