caa a22 4500 378867318 CHVBK 20180305123401.0 cr unu---uuuuu 161128e20030617xx s 000 0 eng 10.1515/CCLM.2003.121 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.121 Molecular Cloning, Sequencing and Functional Expression of Porcine Thyrotropin (TSH) Receptor cDNA [Elektronische Daten] [Makoto Igarashi, Atsuo Nagata] We directly sequenced PCR fragments amplified from a porcine thyroid cDNA library with various synthetic primers based on the nucleotide sequence of bovinethyrotropin receptor (bTSHR) cDNA. A nucleotide sequence of 2410 bp was determined and its deduced amino acid sequence contained an open reading frame coding 764 amino acids, which showed 91.8% and 89.5% similarity to bTSHR and human TSH receptor, respectively. A hydrophobicity plot of the porcine protein suggested that it had a putative signal peptide and seven transmembrane domains. Chinese hamster ovary cells stably transfected with the cDNA could be specifically bound to bovine TSH (bTSH) and increased the intracellular cAMP level in response to bTSH and Graves' disease patients' sera stimulation. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Igarashi Makoto aut Nagata Atsuo aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 796-803 1434-6621 41:6<796 2003 41 cclm https://doi.org/10.1515/CCLM.2003.121 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.121 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Igarashi Makoto aut NATIONALLICENCE 700 1- Nagata Atsuo aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/6(2003-06-17), 796-803 1434-6621 41:6<796 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter