Assessment of Intestinal Permeability: EnzymaticDetermination of Urinary Mannitol, Raffinose,Sucrose and Lactose on Hitachi Analyzer
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| 024 | 7 | 0 | |a 10.1515/CCLM.2003.007 |2 doi |
| 035 | |a (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.007 | ||
| 245 | 0 | 0 | |a Assessment of Intestinal Permeability: EnzymaticDetermination of Urinary Mannitol, Raffinose,Sucrose and Lactose on Hitachi Analyzer |h [Elektronische Daten] |c [Jan Hessels, Ellen J.M. Snoeyink, Antonius J. Platenkamp, Gerrit Voortman, Jan Steggink, Harry H.M. Eidhof] |
| 520 | 3 | |a The sugar absorption test is the usual test for measurement of intestinal permeability. After intestinal absorption of probe sugars the subsequently excreted sugars are measured in urine. We have developed four enzymatic methods for the measurement of the urinary concentration of the probe sugars mannitol, raffinose, lactose and sucrose. Mannitol, lactose and sucrose are directly measured on Hitachi 917 using mannitol dehydrogenase, β-galactosidase and invertase, respectively, as enzyme reagents. Raffinose measurement needs a three hours preincubation with α-galactosidase, after which the liberated sucrose is measured. The analytical performances such as within- and between-run precision, linearity, lowest detection limit, interference of other sugars and comparison with a gas chromatographic method are described for the four methods. These methods are accurate an can easily be performed in any clinical laboratory. | |
| 540 | |a Copyright © 2003 by Walter de Gruyter GmbH & Co. KG | ||
| 690 | 7 | |a Medical equipment & techniques |2 nationallicence | |
| 690 | 7 | |a Medical diagnosis |2 nationallicence | |
| 690 | 7 | |a Diseases & disorders |2 nationallicence | |
| 700 | 1 | |a Hessels |D Jan |4 aut | |
| 700 | 1 | |a Snoeyink |D Ellen J.M. |4 aut | |
| 700 | 1 | |a Platenkamp |D Antonius J. |4 aut | |
| 700 | 1 | |a Voortman |D Gerrit |4 aut | |
| 700 | 1 | |a Steggink |D Jan |4 aut | |
| 700 | 1 | |a Eidhof |D Harry H.M. |4 aut | |
| 773 | 0 | |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/1(2003-01-27), 33-38 |x 1434-6621 |q 41:1<33 |1 2003 |2 41 |o cclm | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/CCLM.2003.007 |q text/html |z Onlinezugriff via DOI |
| 908 | |D 1 |a research article |2 jats | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1515/CCLM.2003.007 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hessels |D Jan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Snoeyink |D Ellen J.M. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Platenkamp |D Antonius J. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Voortman |D Gerrit |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Steggink |D Jan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Eidhof |D Harry H.M. |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Clinical Chemistry and Laboratory Medicine |d Walter de Gruyter |g 41/1(2003-01-27), 33-38 |x 1434-6621 |q 41:1<33 |1 2003 |2 41 |o cclm | ||
| 900 | 7 | |b CC0 |u http://creativecommons.org/publicdomain/zero/1.0 |2 nationallicence | |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-gruyter | ||