caa a22 4500 378869604 CHVBK 20180305123406.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/CCLM.2003.007 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.007 Assessment of Intestinal Permeability: EnzymaticDetermination of Urinary Mannitol, Raffinose,Sucrose and Lactose on Hitachi Analyzer [Elektronische Daten] [Jan Hessels, Ellen J.M. Snoeyink, Antonius J. Platenkamp, Gerrit Voortman, Jan Steggink, Harry H.M. Eidhof] The sugar absorption test is the usual test for measurement of intestinal permeability. After intestinal absorption of probe sugars the subsequently excreted sugars are measured in urine. We have developed four enzymatic methods for the measurement of the urinary concentration of the probe sugars mannitol, raffinose, lactose and sucrose. Mannitol, lactose and sucrose are directly measured on Hitachi 917 using mannitol dehydrogenase, β-galactosidase and invertase, respectively, as enzyme reagents. Raffinose measurement needs a three hours preincubation with α-galactosidase, after which the liberated sucrose is measured. The analytical performances such as within- and between-run precision, linearity, lowest detection limit, interference of other sugars and comparison with a gas chromatographic method are described for the four methods. These methods are accurate an can easily be performed in any clinical laboratory. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Hessels Jan aut Snoeyink Ellen J.M. aut Platenkamp Antonius J. aut Voortman Gerrit aut Steggink Jan aut Eidhof Harry H.M. aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 33-38 1434-6621 41:1<33 2003 41 cclm https://doi.org/10.1515/CCLM.2003.007 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hessels Jan aut NATIONALLICENCE 700 1- Snoeyink Ellen J.M. aut NATIONALLICENCE 700 1- Platenkamp Antonius J. aut NATIONALLICENCE 700 1- Voortman Gerrit aut NATIONALLICENCE 700 1- Steggink Jan aut NATIONALLICENCE 700 1- Eidhof Harry H.M. aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 33-38 1434-6621 41:1<33 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter