caa a22 4500 378869612 CHVBK 20180305123406.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/CCLM.2003.011 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.011 Modified Method to Measure Nasal PotentialDifference [Elektronische Daten] [Teresinha Leal, Jean Lebacq, Patrick Lebecque, Jean Cumps, Pierre Wallemacq] Nasal potential difference (NPD) measurements have been proposed to assess defective ion transport in cystic fibrosis (CF). Implementing it routinely is, however, difficult. Therefore, a modified method based on nasal instillation in supine position at reduced flow rate was tested to evaluate its ability to discriminate CF from non-CF subjects. Classical and modified methods were compared in nine healthy subjects and there were no statistical differences. Following the new method, 97 tests were performed on 74 subjects divided in three cohorts: 21 CF patients and two control groups consisting of 19 patients with other pulmonary diseases and 34 healthy subjects. Twenty five children were enrolled in this study. Maximal NPD in CF patients (−44.9±2.5 mV) was significantly different from that obtained in control groups (−18.1±1.6 and −17.2±1.1 mV). Depolarization after amiloride also discriminated CF patients (25.9±1.4 mV) from control groups (10.5±0.9 and 8.1±0.7 mV). Marked repolarization following isoprenaline plus amiloride in low chloride solution was seen in control groups (−15.7±1.1 and −15.3±1.1 mV). We conclude that the modified method represents a simplified and equally effective approach to discriminate CF patients from non-CF subjects. Moreover, this method presents practical advantages for the patients related to hygiene and convenience, favoring its application in small children. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Leal Teresinha aut Lebacq Jean aut Lebecque Patrick aut Cumps Jean aut Wallemacq Pierre aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 61-67 1434-6621 41:1<61 2003 41 cclm https://doi.org/10.1515/CCLM.2003.011 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leal Teresinha aut NATIONALLICENCE 700 1- Lebacq Jean aut NATIONALLICENCE 700 1- Lebecque Patrick aut NATIONALLICENCE 700 1- Cumps Jean aut NATIONALLICENCE 700 1- Wallemacq Pierre aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 61-67 1434-6621 41:1<61 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter