caa a22 4500 378869639 CHVBK 20180305123406.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/CCLM.2003.003 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.003 Total Oxyradical Scavenging Capacity towardDifferent Reactive Oxygen Species in SeminalPlasma and Sperm Cells [Elektronische Daten] [Giancarlo Balercia, Tatiana Armeni, Franco Mantero, Giovanni Principato, Francesco Regoli] The objective of this work was to evaluate the capability of both seminal plasma and sperm cells to scavenge different forms of oxyradicals and the possible correlation with sperm motility parameters. A total of 14 individuals were analyzed by computer-assisted sperm analysis (CASA) and the results integrated with the measurement of total oxyradical scavenging capacity (TOSC) toward peroxyl radicals, hydroxyl radicals and peroxynitrite in seminal plasma and spermatozoa. TOSC values revealed some significant correlation with kinetic sperm cell parameters, including curvilinear velocity (VCL), straight-line velocity (VSL) and linearity (LIN). A lower antioxidant capacity toward hydroxyl radical was found in the seminal fluid of men with reduced sperm motility. Such correlations were not found with peroxyl radicals and peroxynitrite, neither when TOSC values were analyzed in spermatozoa. The TOSC assay is a useful tool for studying the relationship between oxyradical toxicity and abnormal sperm cell motility. Although further investigations are needed, the data clearly establish different role for various forms of oxyradicals, i.e., hydroxyl radicals, in altering sperm motility. Measurement of TOSC is suggested as a useful means of indicating relationship between reactive oxygen species and sperm cell kinetics in clinical trials and antioxidant-based treatments. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Balercia Giancarlo aut Armeni Tatiana aut Mantero Franco aut Principato Giovanni aut Regoli Francesco aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 13-19 1434-6621 41:1<13 2003 41 cclm https://doi.org/10.1515/CCLM.2003.003 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.003 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Balercia Giancarlo aut NATIONALLICENCE 700 1- Armeni Tatiana aut NATIONALLICENCE 700 1- Mantero Franco aut NATIONALLICENCE 700 1- Principato Giovanni aut NATIONALLICENCE 700 1- Regoli Francesco aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 13-19 1434-6621 41:1<13 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter