caa a22 4500 378869698 CHVBK 20180305123406.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/CCLM.2003.010 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.010 Plasma Lipid Composition and LDL Oxidation [Elektronische Daten] [Patrizia Brizzi, Giancarlo Tonolo, Francesca Carusillo, Mariano Malaguarnera, Mario Maioli, Salvatore Musumeci] Low-density lipoprotein (LDL) oxidation in vivo depends on lipid composition and on plasma antioxidant status. The aim of our study was to investigate the relationship between plasma lipid composition and LDL oxidation and, in particular, to explore whether LDL-cholesterol/triglycerides ratio (LDL-C/TG) and LDL-cholesterol/high-density lipoprotein (HDL)-cholesterol ratio (LDL-C/HDL-C) can be used as predictive parameters of LDL oxidation in vivo. In 87 volunteers over a wide range of age plasma lipids and LDL oxidation were studied. Blood was collected after 12 h overnight fast. LDL oxidation was estimated by the level of conjugated diene (BDC) in the lipid fraction isolated from plasma after gradient ultracentrifugation. The results were expressed as μmol/l (BDC/l) to evaluate the level of oxidized LDL, and as nmol of BDC for mg of LDL-cholesterol (BDC/LDL-C) for the evaluation of LDL oxidation degree. BDC/l correlated significantly with age, total and LDL-C, apolipoprotein B and TG, while BDC/LDL-C negatively correlated with total cholesterol, apolipoprotein B, LDL/TG and LDL/HDL ratios. Age of subjects significantly correlated with total and LDL-C and apolipoprotein B. TG have a significant inverse correlation with HDL-C. Our results support the hypothesis that among the several factors involved in LDL oxidation the most important determinants are LDL/TG. Plasma triglycerides appear to be very important even when circulating cholesterol levels are within normal limits. Moreover, we found that the LDL/HDL ratio is also very important with regard to the putative protective role of HDL against LDL oxidation in vivo. In conclusion, plasma lipid parameters must be evaluated not only for their absolute values but also for their mutual ratios as expression of plasma lipid homeostasis. Both LDL/TG and LDL/HDL ratios can be used as predictive parameters of in vivo LDL oxidation. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Brizzi Patrizia aut Tonolo Giancarlo aut Carusillo Francesca aut Malaguarnera Mariano aut Maioli Mario aut Musumeci Salvatore aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 56-60 1434-6621 41:1<56 2003 41 cclm https://doi.org/10.1515/CCLM.2003.010 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.010 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brizzi Patrizia aut NATIONALLICENCE 700 1- Tonolo Giancarlo aut NATIONALLICENCE 700 1- Carusillo Francesca aut NATIONALLICENCE 700 1- Malaguarnera Mariano aut NATIONALLICENCE 700 1- Maioli Mario aut NATIONALLICENCE 700 1- Musumeci Salvatore aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 56-60 1434-6621 41:1<56 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter