caa a22 4500 37886971X CHVBK 20180305123406.0 cr unu---uuuuu 161128e20030127xx s 000 0 eng 10.1515/CCLM.2003.014 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.014 Urinary Plasma Protein Patterns in AcuteProstatitis [Elektronische Daten] [Karel Everaert, Joris Delanghe, Marc Vanderkelen, Kristof Cornelis, Stefaan De Wachter, Annick Viaene, Eddy De Ruyck, Bart Clarysse, Willem Oosterlinck] We evaluated the diagnostic utility of urinary α1-microglobulin, α2-macroglobulin and albumin in the diagnosis of acute prostatitis. We studied 133 men (43±17 years) with, and a reference population (n=36, 41±16 years) without, urinary tract infection. Prostatectomy samples were used to study the potential interference between prostatic proteins and protein analysis. Urinary α2-macroglobulin/albumin ratio was significantly lower in prostatitis compared to the reference population, cystitis or acute pyelonephritis (p <0.0001). Low α2-macroglobulin concentrations in prostatitis are due to inhibition (p = 0.0001) of the immune reaction between α2-macroglobulin in presence of polyclonal rabbit antibodies (used for immunonephelometry) by soluble prostatic proteins (± 60 kDa) which appear in urine in acute prostatitis. The urinary α1-microglobulin/creatinine ratio diagnoses acute pyelonephritis (sensitivity 100% and specificity 87%) and the urinary α2-macroglobulin/albumin ratio diagnoses acute prostatitis (sensitivity 100% and specificity of 90%). Stepwise multinomial logistic regression analysis reveals that urinary α1-microglobulin, α2-macroglobulin, albumin and creatinine provide optimal differentiation between acute pyelonephritis and acute prostatitis (pseudo R2=0.83; Loglikelihood −30.55, p <0.000001). In conclusion, the combination of hematuria and absence of urinary alpha-2-macroglobulin is diagnostic for acute prostatitis. Even without hematuria, α2-macroglobulin remains lower compared to patients without prostatitis. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Everaert Karel aut Delanghe Joris aut Vanderkelen Marc aut Cornelis Kristof aut De Wachter Stefaan aut Viaene Annick aut De Ruyck Eddy aut Clarysse Bart aut Oosterlinck Willem aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 79-84 1434-6621 41:1<79 2003 41 cclm https://doi.org/10.1515/CCLM.2003.014 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.014 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Everaert Karel aut NATIONALLICENCE 700 1- Delanghe Joris aut NATIONALLICENCE 700 1- Vanderkelen Marc aut NATIONALLICENCE 700 1- Cornelis Kristof aut NATIONALLICENCE 700 1- De Wachter Stefaan aut NATIONALLICENCE 700 1- Viaene Annick aut NATIONALLICENCE 700 1- De Ruyck Eddy aut NATIONALLICENCE 700 1- Clarysse Bart aut NATIONALLICENCE 700 1- Oosterlinck Willem aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/1(2003-01-27), 79-84 1434-6621 41:1<79 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter