caa a22 4500 378872494 CHVBK 20180305123412.0 cr unu---uuuuu 161128e20030707xx s 000 0 eng 10.1515/BOT.2003.035 doi (NATIONALLICENCE)gruyter-10.1515/BOT.2003.035 Preparation and Chemical Characterization of Cell Wall Fractions Enriched in Structural Proteinsfrom Palmaria palmata (Rhodophyta) [Elektronische Daten] [E. Deniaud, J. Fleurence, M. Lahaye] A protocol using physical, chemical and enzymatic treatments was developed to prepare cell wall fractions enriched in structural proteins from the red alga Palmaria palmata. The walls obtained from cortical and medullary cells of soft and rigid thalli were chemically characterized. They were all rich in proteins (29-36%DW) and contained ash (4-34%DW), xylose (6-19%DW), glucose (5-13%DW) and galactose (1%DW). Fractions from cortical cells were richer in ash, while those from medullary cells were richer in xylose. The amino acid composition of the fractions differed from that of the initial starting algal materials. They were enriched in tyrosine and methionine, which could indicate peculiar protein cross-links such as phenolic couplings and/or disulfur bridges. Extraction of these fractions by 2 M NaCl isolated minor amounts of materials (approximately 10%DW of cell wall fractions) containing glucose, xylose, proteins, galactose and sulfates. The protein-rich insoluble residues were partially degraded by bromelain, papain, trypsin and chymotrypsin into minor amounts of proteic fractions, indicating that the proteases have a restricted access to the cell wall proteins, probably as a result of high proportions of cross-links. Some fragments contained proteins and xylose, galactose and glucose, suggesting the existence of glycoside side-chains on proteins and/or linkages between cell wall polysaccharides and proteins. The difference in the composition and structure of proteins in the cell wall fractions from soft vs. rigid thalli, and from cortical vs. medullary cells demonstrated that these cell wall structures are closely regulated in relation to cell development and specialization. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Hydrobiology nationallicence Mycology, fungi nationallicence Botany & plant sciences nationallicence Deniaud E. aut Fleurence J. aut Lahaye M. aut Botanica Marina Walter de Gruyter 46/4(2003-07-07), 366-377 0006-8055 46:4<366 2003 46 botm https://doi.org/10.1515/BOT.2003.035 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BOT.2003.035 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deniaud E. aut NATIONALLICENCE 700 1- Fleurence J. aut NATIONALLICENCE 700 1- Lahaye M. aut NATIONALLICENCE 773 0- Botanica Marina Walter de Gruyter 46/4(2003-07-07), 366-377 0006-8055 46:4<366 2003 46 botm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter