caa a22 4500 378874225 CHVBK 20180305123416.0 cr unu---uuuuu 161128e20030220xx s 000 0 eng 10.1515/BC.2003.029 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.029 Quantitation of Membrane Type SerineProtease 1 (MT-SP1) in Transformed andNormal Cells [Elektronische Daten] [A.S. Bhatt, T. Takeuchi, B. Ylstra, D. Ginzinger, D. Albertson, M.A. Shuman, C.S. Craik] Membrane type serine protease 1 (MT-SP1) is a representative member of a large family of related enzymes known as type II transmembrane serine proteases or membrane type serine proteases. MTSP1 has been implicated in the selective proteolysis of key extracellular substrates but its physiological role is still not fully understood. MT-SP1 expression at the protein and RNA level has been previously examined by nonquantitative methods such as in situ hybridization, Northern blotting and immunohistochemistry. To establish an introductory understanding of the quantitative mRNA expression of MT-SP1 and to correlate these levels with urokinasetype plasminogen activator receptor (uPAR), a key component of extracellular proteolysis, quantitative RT-PCR was carried out. RNA expression was analyzed in 34 human cancer cell lines, 26 human tissues and 18 primary human breast cancer tissue samples. MT-SP1 mRNA is highly expressed in many breast, ovarian, prostate and colon cancer cell lines and normal human tissues of endodermal origin. At the transcript level, MT-SP1 shows a highly statistically significant correlation (Pearsons product moment correlation r = 0.784, p < 0.001) with uPAR in human breast cancer tissue. The exact role of MT-SP1 in concert with proteins such as uPAR and other members of the plasminogen activator cascade has yet to be ascertained. However, the significant correlation between MT-SP1 and uPAR transcript levels in this initial study suggests further work to establish the role of MT-SP1 as a possible prognostic, diagnostic or therapeutic target for breast cancer. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Bhatt A.S. aut Takeuchi T. aut Ylstra B. aut Ginzinger D. aut Albertson D. aut Shuman M.A. aut Craik C.S. aut Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 257-266 1431-6730 384:2<257 2003 384 bchm https://doi.org/10.1515/BC.2003.029 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.029 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhatt A.S. aut NATIONALLICENCE 700 1- Takeuchi T. aut NATIONALLICENCE 700 1- Ylstra B. aut NATIONALLICENCE 700 1- Ginzinger D. aut NATIONALLICENCE 700 1- Albertson D. aut NATIONALLICENCE 700 1- Shuman M.A. aut NATIONALLICENCE 700 1- Craik C.S. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 257-266 1431-6730 384:2<257 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter