caa a22 4500 37887425X CHVBK 20180305123416.0 cr unu---uuuuu 161128e20030220xx s 000 0 eng 10.1515/BC.2003.027 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.027 Different Isoforms of the Non-Integrin LamininReceptor Are Present in Mouse Brain and BindPrP [Elektronische Daten] [S. Simoneau, S. Haïk, C. Leucht, D. Dormont, J.-P. Deslys, S. Weiss, C. Lasmézas] The prion protein (PrP) plays a central role in prion diseases, and identifying its cellular receptor appears to be of crucial interest. We previously showed in the yeast two-hybrid system that PrP interacts with the 37 kDa precursor (LRP) of the high affinity 67 kDa laminin receptor (LR), which acts as the cellular receptor of PrP in cellular models. However, among the various isoforms of the receptor that have been identified so far, those which are present in the central nervous system and which bind PrP are still unknown. In this study, we have purified mouse brain fractions enriched in the laminin receptor and have performed overlay assays in order to identify those isoforms that interact with the prion protein. We demonstrate (i) the presence, in mouse brain, of several isoforms of the LRP/LR corresponding to different maturation states of the receptor (44, 60, 67 and 220 kDa) and (ii) the binding of all of these isoforms to PrP. Our data strongly support a physiological role of the laminin receptor/ PrP interaction in the brain and highlight its relevance for transmissible spongiform encephalopathies. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Simoneau S. aut Haïk S. aut Leucht C. aut Dormont D. aut Deslys J.-P aut Weiss S. aut Lasmézas C. aut Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 243-246 1431-6730 384:2<243 2003 384 bchm https://doi.org/10.1515/BC.2003.027 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.027 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Simoneau S. aut NATIONALLICENCE 700 1- Haïk S. aut NATIONALLICENCE 700 1- Leucht C. aut NATIONALLICENCE 700 1- Dormont D. aut NATIONALLICENCE 700 1- Deslys J.-P aut NATIONALLICENCE 700 1- Weiss S. aut NATIONALLICENCE 700 1- Lasmézas C. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 243-246 1431-6730 384:2<243 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter