caa a22 4500 378874276 CHVBK 20180305123416.0 cr unu---uuuuu 161128e20030220xx s 000 0 eng 10.1515/BC.2003.032 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.032 A Homodimeric Sporamin-Type TrypsinInhibitor with Antiproliferative, HIV ReverseTranscriptase-Inhibitory and AntifungalActivities from Wampee (Clausena lansium)Seeds [Elektronische Daten] [T.B. Ng, S.K. Lam, W.P. Fong] A homodimeric trypsin inhibitor with a molecular mass of 54 kDa was isolated from the seeds of Clausena lansium (Lour) Skeels with a very simple procedure comprising extraction with an aqueous buffer and ion exchange chromatography on CM-cellulose. It inhibited trypsin with an IC50 of 2.2 nM but was without any inhibitory effect on chymotrypsin and proteinase K. The uptake of MTT by human leukemia HL60 and hepatoma Hep G2 cells was inhibited with an IC50 of 100 uM. Translation in the cellfree rabbit reticulocyte lysate system was inhibited with an IC50 of 3.6 uM. The activity of HIV-1 reverse transcriptase was reduced in the presence of the trypsin inhibitor. The trypsin inhibitor exerted antifungal activity toward Physalospora piricola but not Mycosphaerella arachidicola, Botrytis cinerea, Fusarium oxysporum or Coprinus comatus. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Ng T.B. aut Lam S.K. aut Fong W.P. aut Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 289-293 1431-6730 384:2<289 2003 384 bchm https://doi.org/10.1515/BC.2003.032 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.032 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ng T.B. aut NATIONALLICENCE 700 1- Lam S.K. aut NATIONALLICENCE 700 1- Fong W.P. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 289-293 1431-6730 384:2<289 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter