caa a22 4500 378874292 CHVBK 20180305123416.0 cr unu---uuuuu 161128e20030220xx s 000 0 eng 10.1515/BC.2003.028 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.028 Cell-Type Specific Targeting and GeneExpression Using a Variant of Polyoma VP1Virus-Like Particles [Elektronische Daten] [S. Gleiter, H. Lilie] The variant VP1-Z of the polyomavirus coat protein VP1 has been recently described as an engineered fusion protein of VP1 and the antibody binding domain protein Z. This construct is able to specifically bind and functionally present antibodies on the surface of viruslike particles of VP1-Z. Here we demonstrate that with the binding of Herceptin, an antibody directed against the receptor tyrosine kinase ErbB2, a cell typespecific targeting was established. ErbB2-positive cell lines were transduced with different plasmids encoding eGFP or β-galactosidase. With both reporter systems functional gene expression in transduced cells could be observed. The transduction was strictly dependent on the use of a ternary complex formed of VLPs of VP1- Z, Herceptin, and the reporter plasmid DNA. The use of single components or ErbB2-negative cell lines did not result in functional gene transfer. The transduction was also completely dependent on the use of chloroquine, a lysosomotropic reagent. This indicates that the complex is internalized by ErbB2-mediated endocytosis. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Gleiter S. aut Lilie H. aut Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 247-255 1431-6730 384:2<247 2003 384 bchm https://doi.org/10.1515/BC.2003.028 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.028 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gleiter S. aut NATIONALLICENCE 700 1- Lilie H. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 247-255 1431-6730 384:2<247 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter