caa a22 4500 378874365 CHVBK 20180305123417.0 cr unu---uuuuu 161128e20030220xx s 000 0 eng 10.1515/BC.2003.033 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.033 Primary Structure and Reactive Site of a NovelWheat Proteinase Inhibitor of Subtilisin andChymotrypsin [Elektronische Daten] [E. Poerio, S. Di Gennaro, A. Di Maro, F. Farisei, P. Ferranti, A. Parente] The proteinase inhibitor WSCI, active in inhibiting bacterial subtilisin and a number of animal chymotrypsins, was purified from endosperm of exaploid wheat (Triticum aestivum, c.v. San Pastore) by ion exchange chromatography and its complete amino acid sequence was established by automated Edman degradation. WSCI consists of a single polypeptide chain of 72 amino acid residues, has a molecular mass of 8126.3 Da and a pI of 5.8. The inhibition constants (K1) for Bacillus licheniformis subtilisin and bovine pancreatic α-chymotrypsin are 3.92×10 -9 M and 7.24×10 -9 M, respectively. The inhibitor contains one methionine and of tryptophan residue and has a high content of essential amino acids (41 over a total of 72 residues), but no cysteines. The primary structure of WSCI shows high similarity with barley subtilisin-chymotrypsin isoinhibitors of the CI-2 type and with maize subtilisinchymotrypsin inhibitor MPI. Significant degrees of similarity were also found between sequences of WSCI and of other members of the potato inhibitor I family of the serine proteinase inhibitors. The wheat inhibitor WSCI has a single reactive site (the peptide bond between methionyl-48 and glutamyl-49 residues) as identified by affinity chromatography and sequence analysis. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Poerio E. aut Gennaro S. Di aut Maro A. Di aut Farisei F. aut Ferranti P. aut Parente A. aut Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 295-304 1431-6730 384:2<295 2003 384 bchm https://doi.org/10.1515/BC.2003.033 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.033 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poerio E. aut NATIONALLICENCE 700 1- Gennaro S. Di aut NATIONALLICENCE 700 1- Maro A. Di aut NATIONALLICENCE 700 1- Farisei F. aut NATIONALLICENCE 700 1- Ferranti P. aut NATIONALLICENCE 700 1- Parente A. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 295-304 1431-6730 384:2<295 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter