caa a22 4500 378874373 CHVBK 20180305123417.0 cr unu---uuuuu 161128e20030220xx s 000 0 eng 10.1515/BC.2003.022 doi (NATIONALLICENCE)gruyter-10.1515/BC.2003.022 Transcriptional Repression of the Human p53Gene by Hepatitis B Viral Core Protein (HBc) inHuman Liver Cells [Elektronische Daten] [J.A. Kwon, H.M. Rho] Hepatitis B virus (HBV) is a causative agent of chronic and acute hepatitis, and is associated with the development of hepatocellular carcinoma (HCC). We demonstrate here that the Hepatitis B viral core protein (HBc) functions as a repressor on the promoter activity of the human p53 gene. The functional analyses of the promoter of the p53 gene by serial deletion, sitedirected mutagenesis, and the heterologous promoter system revealed that the promoter activity was repressed through the E2F1-binding site (nucleotides -28 to -8) by HBc. An electrophoretic mobility shift assay (EMSA) showed that the HBc reduced the DNA-binding ability of E2F1 to the binding site of the p53 promoter. The interaction of HBc with E2F1 was also observed by glutathione S-transferase (GST) fusion protein binding assay. Furthermore, HBc represses the expression of the p53 gene in the human liver cell line HepG2. Finally, HBc and HBx synergistically repress both the promoter activity and the expression of the p53 gene in HepG2 cells. These results, together with our previous study, strongly suggest that HBc, like HBx, represses the expression of the human p53 tumor suppressor gene. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Biochemistry nationallicence Molecular biology nationallicence Cellular biology nationallicence Kwon J.A. aut Rho H.M. aut Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 203-212 1431-6730 384:2<203 2003 384 bchm https://doi.org/10.1515/BC.2003.022 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/BC.2003.022 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kwon J.A. aut NATIONALLICENCE 700 1- Rho H.M. aut NATIONALLICENCE 773 0- Biological Chemistry Walter de Gruyter 384/2(2003-02-20), 203-212 1431-6730 384:2<203 2003 384 bchm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter