caa a22 4500 378875981 CHVBK 20180305123420.0 cr unu---uuuuu 161128e20030515xx s 000 0 eng 10.1515/CCLM.2003.106 doi (NATIONALLICENCE)gruyter-10.1515/CCLM.2003.106 Measurements of Serum S-100B Protein: Effects ofStorage Time and Temperature on Pre-AnalyticalStability [Elektronische Daten] [Andreas Raabe, Olaf Kopetsch, Ulrich Groß, Michael Zimmermann, Peter Gebhart] Measurement of S-100B protein in serum or cerebrospinal fluid is increasingly used as a molecular marker of brain damage. However, there is no information about the pre-analytical in vitro stability of S-100B. We have investigated whether storing blood samples at different time periods and conditions affected the measured levels of S-100B protein. Blood samples were taken from 29 neurosurgical patients with normal serum S-100B values and from seven patients with increased serum S-100B values. Blood samples were immediately divided into nine aliquots for measuring S-100B immediately and after 4, 8, 12, and 24 hours, stored at room temperature or at 4 °C. Measurement of S-100B was performed using the LIAISON® assay (Byk-Sangtec Diagnostica, Dietzenbach, Germany). Moreover, in 10 additional patients the effect of freezing the serum and thawing the sample after 24 hours was investigated. There were no differences between the results of S-100B measurements after storing the sample at different temperatures and time periods. There was no trend towards higher or lower values in all three groups. Therefore, blood samples may be collected as part of the daily clinical routine without time constraint and even stored overnight without affecting S-100B serum levels when measured with the LIAISON® Sangtec® 100 assay. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG Medical equipment & techniques nationallicence Medical diagnosis nationallicence Diseases & disorders nationallicence Raabe Andreas aut Kopetsch Olaf aut Groß Ulrich aut Zimmermann Michael aut Gebhart Peter aut Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/5(2003-05-15), 700-703 1434-6621 41:5<700 2003 41 cclm https://doi.org/10.1515/CCLM.2003.106 text/html Onlinezugriff via DOI 1 research article jats NATIONALLICENCE 856 40 https://doi.org/10.1515/CCLM.2003.106 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raabe Andreas aut NATIONALLICENCE 700 1- Kopetsch Olaf aut NATIONALLICENCE 700 1- Groß Ulrich aut NATIONALLICENCE 700 1- Zimmermann Michael aut NATIONALLICENCE 700 1- Gebhart Peter aut NATIONALLICENCE 773 0- Clinical Chemistry and Laboratory Medicine Walter de Gruyter 41/5(2003-05-15), 700-703 1434-6621 41:5<700 2003 41 cclm CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-gruyter